| Mild hypothermia protects against postischemic hepatic endothelial injury and decreases the formation of reactive oxygen species. | |
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MedLine Citation:
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PMID: 11145106 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The ability of mild hypothermia (MH; 34 degrees C) to protect against postischemic endothelial injury and decrease reactive oxygen species' (ROS) formation was studied using lucigenin and luminol enhanced chemiluminescence (CL). Lucigenin CL is largely specific for superoxide, while luminol reacts with many ROS. Isolated rat livers perfused under constant flow in a non-recirculating system were exposed to 2.5 h of ischemia after 0.5 h perfusion with Krebs-Henseleit buffer at either normothermia (38 degrees C) or mild hypothermia (34 degrees C) (n = 5, all groups). CL (cps), vascular resistance (Woods units), O2 consumption, and potassium efflux were measured at the end of perfusion, and at 0 min reperfusion, and every 30 min during reperfusion. For both the lucigenin and luminol groups, CL and vascular resistance increased significantly (repeat measures ANOVA, P <0.05) for normothermia (NT, 38 degrees C) but not mild hypothermia. Potassium efflux did not change significantly for the mild hypothermia groups. In the luminol enhanced group, oxygen consumption was greater in the mildly hypothermic group at 1 h and 1.5 h of reperfusion. Mild hypothermia decreased postischemic ROS production. Increased vascular resistance in the normothermia group may indicate an endothelial injury. Mild hypothermia appears to protect against this injury. |
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Authors:
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H A Zar; J R Lancaster |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Redox report : communications in free radical research Volume: 5 ISSN: 1351-0002 ISO Abbreviation: Redox Rep. Publication Date: 2000 |
Date Detail:
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Created Date: 2000-12-28 Completed Date: 2001-02-22 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 9511366 Medline TA: Redox Rep Country: England |
Other Details:
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Languages: eng Pagination: 303-10 Citation Subset: IM |
Affiliation:
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Department of Anesthesiology, University of North Carolina, Chapel Hill 27599, USA. haz1859@med.unc.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acridines Animals Chemiluminescent Measurements Endothelium, Vascular / metabolism* Free Radicals / metabolism Hypothermia, Induced* Indicators and Reagents Liver / blood supply, metabolism* Luminol Male Rats Rats, Sprague-Dawley Reactive Oxygen Species / metabolism* Reperfusion Injury / prevention & control* Superoxides / metabolism Vascular Resistance |
| Grant Support | |
ID/Acronym/Agency:
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DK46935-05/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Acridines; 0/Free Radicals; 0/Indicators and Reagents; 0/Reactive Oxygen Species; 11062-77-4/Superoxides; 2315-97-1/10,10'-dimethyl-9,9'-biacridinium; 521-31-3/Luminol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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