Document Detail


Mild hypotension and body burns synergistically increase bacterial translocation in rats consistent with a "two-hit phenomenon".
MedLine Citation:
PMID:  9063783     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Wistar rats were burned over 15% of their total body surface area and suffered hemorrhagic hypotension (mean blood pressure 80 mm Hg) 72 hours after the thermal injury (burn + hypotension group). Rats in other groups were treated in the same way except sham hypotension in burn group, sham burn in hypotension group, and sham hypotension and sham burn in control group. At the end of the study period, the mesenteric lymph nodes (MLN), liver, and spleen were cultured for translocated bacteria. The cecal bacterial burden was not affected by the insults. The number of bacteria translocated to the MLN after the thermal injury was much greater in the burn + hypotension group (156.19 +/- 42.29 colony-forming units [CFUs]/gm tissue) rather than in the other groups (5.24 +/- 8.34, 9.86 +/- 16.56, 40.21 +/- 45.8 in the control, hypotension, and burn groups, respectively). This indicates that bacterial translocation (BT) is the result of a two-hit phenomenon. The gut-MLN BT ratio, obtained by dividing the bacterial CFUs in the MLN by those in the cecum, was higher in the burn + hypotension group than in the other groups (p < 0.01). This ratio allows standardized measurements of BT to the MLN despite differences in the absolute bacterial load in the gut, and the elevated gut mesenteric lymph nodes BT ratio of the burn + hypotension group suggests that the two-hit phenomenon of BT may be the result of alterations in the gut mucosal barrier function.
Authors:
S Mishima; T Yukioka; H Matsuda; S Shimazaki
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of burn care & rehabilitation     Volume:  18     ISSN:  0273-8481     ISO Abbreviation:  J Burn Care Rehabil     Publication Date:    1997 Jan-Feb
Date Detail:
Created Date:  1997-05-23     Completed Date:  1997-05-23     Revised Date:  2006-08-15    
Medline Journal Info:
Nlm Unique ID:  8110188     Medline TA:  J Burn Care Rehabil     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  22-6     Citation Subset:  IM    
Affiliation:
Department of Traumatology and Critical Care Medicine, Kyorin University, Tokyo.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacterial Translocation*
Burns / complications,  microbiology*
Colony Count, Microbial
Hypotension / complications*
Liver / microbiology
Lymph Nodes / microbiology
Male
Mesentery
Rats
Rats, Wistar
Shock, Hemorrhagic / complications,  microbiology
Spleen / microbiology

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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