| Mild detritylation of nucleic acid hydroxyl groups by warming up. | |
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MedLine Citation:
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PMID: 21623541 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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It is challenging to effectively deprotect hydroxyl groups of acid-or-base sensitive bio-macromolecules without causing even minor defects and compromising high quality of final products. We report here a mild detritylation strategy in mildly acidic buffers to remove the DMTr protection from the 5'-hydroxyl groups of synthetic nucleic acids. The DMTr-groups can be easily and effectively removed at pH 4.5 or 5.0 with slight warming up (40 °C), offering virtually quantitative deprotection. This warming-up strategy is particularly useful for deprotection of the modified nucleic acids that are sensitive to the conventional acid deprotection. As a first step towards our long-term goal of synthesizing defect-free nucleic acids, our novel and simple strategy further increases the quality of synthetic nucleic acids. |
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Authors:
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Jozef Salon; Bo Zhang; Zhen Huang |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Nucleosides, nucleotides & nucleic acids Volume: 30 ISSN: 1532-2335 ISO Abbreviation: Nucleosides Nucleotides Nucleic Acids Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-05-30 Completed Date: 2011-11-15 Revised Date: 2012-04-04 |
Medline Journal Info:
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Nlm Unique ID: 100892832 Medline TA: Nucleosides Nucleotides Nucleic Acids Country: England |
Other Details:
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Languages: eng Pagination: 271-9 Citation Subset: IM |
Affiliation:
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Department of Chemistry, Georgia State University, Atlanta, Georgia 30303, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Base Sequence DNA / chemistry*, genetics Hot Temperature* Hydrogen-Ion Concentration Hydroxides / chemistry* RNA / chemistry*, genetics Trityl Compounds / chemistry* |
| Grant Support | |
ID/Acronym/Agency:
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GM095086/GM/NIGMS NIH HHS; R41 GM095086-01/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Hydroxides; 0/Trityl Compounds; 14280-30-9/hydroxide ion; 63231-63-0/RNA; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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