Document Detail


Migratory patterns of thoracic duct lymphocytes into bronchus-associated lymphoid tissue of immunized rats.
MedLine Citation:
PMID:  11147313     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Lymphocytes continuously circulate between the bloodstream and lymphoid organs, and their migration into lymphatic tissues presumably occurs through selective mechanisms. Although bronchus-associated lymphoid tissue (BALT) is known as an inductive tissue of the common mucosal immune system, little is known about how effectively the lymphocytes in the blood vessels migrate into the BALT, thereby enabling the BALT to act as an effector tissue in the immunologic responses of the lungs. To analyze whether or not thoracic duct lymphocytes (TDL) from immunized and nonimmunized rats possess different migratory patterns to the BALT, 5-(6)-carboxyfluorescein diacetate succinimidyl ester (CFSE)-labeled TDL were injected into rats with BALT hyperplasia that was produced by intratracheal administration of TNP-KLH, and then the number of labeled cells in the BALT were examined by immunohistochemical methods. We studied the following three groups at 12 h after the injection: group A, intraintestinally immunized donors and intratracheally immunized recipients; group B, nonimmunized donors and intratracheally immunized recipients; group C (control group), nonimmunized donors and nonimmunized recipients. Time course studies 0.5, 4, 12, and 24 h after the injection were done in groups A and C. In a cytokinetic study, larger numbers of CFSE-labeled lymphocytes were found at 12 h and 24 h in group A than in group C. At 12 h after the injection, the absolute number of CFSE-labeled lymphocytes per BALT was significantly higher in group A than in group B (p < 0.05), and was lowest in group C. Histologically, there was a marked proliferation of high endothelial venules (HEV), with findings of adhesion and influx of lymphocytes inside the HEV in group A. These observations indicate that the immunized BALT actively recruits immunized TDL through a specific mechanism of lymphocyte-endothelium recognition in HEV, which partially explains the process of BALT development as an effector tissue for local immunity.
Authors:
J Sato; K Chida; T Suda; A Sato; H Nakamura
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Lung     Volume:  178     ISSN:  0341-2040     ISO Abbreviation:  Lung     Publication Date:  2000  
Date Detail:
Created Date:  2001-01-05     Completed Date:  2001-02-22     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  7701875     Medline TA:  Lung     Country:  United States    
Other Details:
Languages:  eng     Pagination:  295-308     Citation Subset:  IM    
Affiliation:
Second Division, Department of Internal Medicine, Hamamatsu University School of Medicine, Hamamatsu, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, T-Independent / immunology
Bronchi / blood supply,  cytology*,  immunology
Bronchoalveolar Lavage Fluid / cytology
Cell Movement / physiology*
Endothelium, Vascular / cytology,  physiology
Fluoresceins
Fluorescent Dyes
Hemocyanin / immunology
Immunity, Mucosal
Immunization
Lymphocyte Count
Lymphocytes / physiology*
Lymphoid Tissue / blood supply,  cytology*,  immunology
Male
Rats
Succinimides
Thoracic Duct / blood supply,  cytology*
Chemical
Reg. No./Substance:
0/5-(6)-carboxyfluorescein diacetate succinimidyl ester; 0/Antigens, T-Independent; 0/Fluoresceins; 0/Fluorescent Dyes; 0/Succinimides; 0/trinitrophenyl keyhole limpet hemocyanin; 9013-72-3/Hemocyanin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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