Document Detail


Migration of turkey muscle satellite cells is enhanced by the syndecan-4 cytoplasmic domain through the activation of RhoA.
MedLine Citation:
PMID:  23212449     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Syndecan-4 (S4) is a cell membrane-associated heparan sulfate proteoglycan that forms oligomers in muscle satellite cells. The S4 oligomers activate protein kinase Cα (PKCα) through the S4 cytoplasmic domain and may regulate the activation of ras homolog gene family member A (RhoA), a signal transduction molecule down-stream of PKCα which is thought to influence cell migration. However, little is known about the function of the S4 cytoplasmic domain in satellite cell migration and RhoA activation. The objective of the current study was to determine the function of S4 and its cytoplasmic domain in cell migration and RhoA activation. To study the objective, clones of S4 and S4 without the cytoplasmic domain (S4C) were used in overexpression studies, and small interference RNAs targeting S4 or RhoA were used in knockdown studies. Satellite cell migration was increased by S4 overexpression, but decreased by the knockdown or deletion of the S4 cytoplasmic domain. The RhoA protein was activated by the overexpression of S4, but not with the deletion of the S4 cytoplasmic domain. The treatment of Rho activator II or the knockdown of RhoA also modulated satellite cell migration. Finally, co-transfection (S4 overexpression and RhoA knockdown) and rescue (the knockdown of S4 and the treatment with Rho activator II) studies demonstrated that S4-mediated satellite cell migration was regulated through the activation of RhoA. The cytoplasmic domain of S4 is required for cell migration and RhoA activation which will affect muscle fiber formation.
Authors:
Jonghyun Shin; Douglas C McFarland; Sandra G Velleman
Related Documents :
23418619 - Gl3, a novel 4β-anilino-4'-o-demethyl-4-desoxypodophyllotoxin analog, traps topoisomer...
24049099 - In search of nucleus pulposus-specific molecular markers.
24439289 - Combinatorial screening of mesenchymal stem cell adhesion and differentiation using pol...
24349309 - Exposure to atmospheric particulate matter enhances th17 polarization through the aryl ...
22275199 - In the spotlight: tissue engineering.
23109849 - High mitochondrial dna copy number and bioenergetic function are associated with tumor ...
20015659 - Expansion of beta-cell mass in response to pregnancy.
8975919 - Effects of differential expression of the 49-kilodalton exoenzyme s by pseudomonas aeru...
8166459 - Elutriation of t-lymphoblastic cells and cell cycle analysis of fractions.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-12-5
Journal Detail:
Title:  Molecular and cellular biochemistry     Volume:  -     ISSN:  1573-4919     ISO Abbreviation:  Mol. Cell. Biochem.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0364456     Medline TA:  Mol Cell Biochem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Animal Sciences, Ohio Agricultural Research and Development Center, The Ohio State University, 213 Gerlaugh Hall, 1680 Madison Avenue, Wooster, OH, 44691, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Alcohol-induced oxidative/nitrosative stress alters brain mitochondrial membrane properties.
Next Document:  PTPN12 controls PTEN and the AKT signalling to FAK and HER2 in migrating ovarian cancer cells.