| Migration of rat RNA polymerase I into chick erythrocyte nuclei undergoing reactivation in chick-rat heterokaryons. | |
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MedLine Citation:
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PMID: 6355125 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Transcriptionally inactive chick erythrocyte nuclei were reactivated by Sendai virus-induced fusion of erythrocytes with rat L6J1 myoblasts. We used antibodies to trace the appearance of a specific protein engaged in transcription of a defined class of genes, those coding for rRNA, during reactivation. Using immunofluorescence microscopy, we found increasing amounts of rat RNA polymerase I to appear, during a certain period of time after fusion, in the reforming nucleoli of the chick nuclei. Amounts of rat RNA polymerase I sufficient to be detected by immunofluorescence microscopy had accumulated in the newly developed chick nucleoli 72-190 h after fusion was initiated. This time interval coincides with the time when chick rRNA synthesis can first be detected. The results raise the possibility that during these stages of the reactivation process chick rRNA genes are transcribed by heterologous RNA polymerase I molecules of rat origin. |
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Authors:
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U Scheer; G Lanfranchi; K M Rose; W W Franke; N R Ringertz |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of cell biology Volume: 97 ISSN: 0021-9525 ISO Abbreviation: J. Cell Biol. Publication Date: 1983 Nov |
Date Detail:
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Created Date: 1983-12-17 Completed Date: 1983-12-17 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0375356 Medline TA: J Cell Biol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1641-3 Citation Subset: IM |
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Nucleus / enzymology* Cells, Cultured Chickens Erythrocytes / ultrastructure* Fluorescent Antibody Technique Hybrid Cells / ultrastructure Microscopy, Fluorescence RNA Polymerase I / metabolism* Rats Time Factors |
| Grant Support | |
ID/Acronym/Agency:
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GM 26740/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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EC 2.7.7.-/RNA Polymerase I |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): J Cell Biol ISSN: 0021-9525 ISSN: 1540-8140 Publisher: The Rockefeller University Press |
Article Information Download PDF  Print publication date: Day: 1 Month: 11 Year: 1983 Volume: 97 Issue: 5 First Page: 1641 Last Page: 1643 ID: 2112706 Publisher Id: 84032686 PubMed Id: 6355125 |
| Migration of rat RNA polymerase I into chick erythrocyte nuclei undergoing reactivation in chick-rat heterokaryons | |
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