Document Detail

Migration and multipotentiality of PSA-NCAM+ neural precursors transplanted in the developing brain.
MedLine Citation:
PMID:  11414788     Owner:  NLM     Status:  MEDLINE    
By optimizing the previously described strategy for obtention of spheres enriched in PSA-NCAM+ precursors, we prepared PSA-NCAM-immunoselected cell populations from cerebral hemispheres of neonatal MBP-LacZ transgenic mice. These cells expressed Nestin, exhibited clonal expansion potential and formed spheres, which were initially enriched in PSA-NCAM+ cells but became enriched in GD3+ oligodendrocyte progenitors after 1 week in B104 contionned medium. One month after their periventricular transplantation into the brain of wild-type and/or shiverer newborn mice, cells from PSA-NCAM+ spheres exhibited a higher rostral migration potential than cells from GD3+ spheres, and clearly contributed to myelination in the olfactory bulb. In shiverer hosts, both sphere populations generated oligodendrocytes with similar myelination potential. In addition PSA-NCAM+ sphere cells generated GFAP+ astrocytes and NeuN+ neurons, depending on their site of insertion. These results evidence the high plasticity of newborn PSA-NCAM+ neural precursors and suggest that they are promising tools for cell therapy of CNS diseases, including myelin disorders.
S Vitry; V Avellana-Adalid; F Lachapelle; A Baron-Van Evercooren
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular and cellular neurosciences     Volume:  17     ISSN:  1044-7431     ISO Abbreviation:  Mol. Cell. Neurosci.     Publication Date:  2001 Jun 
Date Detail:
Created Date:  2001-06-20     Completed Date:  2001-08-30     Revised Date:  2013-03-15    
Medline Journal Info:
Nlm Unique ID:  9100095     Medline TA:  Mol Cell Neurosci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  983-1000     Citation Subset:  IM    
Copyright Information:
Copyright 2001 Academic Press.
Institut National de la Santé et de la Recherche Médicale U546, CHU Pitié-Salpêtrière, Paris cedex 13, 75634, France.
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MeSH Terms
Antigens, Differentiation / metabolism
Astrocytes / cytology,  metabolism
Brain / cytology,  growth & development*,  metabolism
Brain Tissue Transplantation / methods
Cell Aggregation / genetics
Cell Culture Techniques / methods
Cell Differentiation / physiology*
Cell Lineage / physiology*
Cell Movement / physiology*
Cells, Cultured / cytology,  drug effects,  metabolism
Central Nervous System Diseases / surgery
Clone Cells / cytology,  drug effects,  metabolism
Culture Media / pharmacology
Gangliosides / metabolism
Gene Expression Regulation, Developmental / physiology
Glial Fibrillary Acidic Protein / metabolism
Graft Survival / physiology
Intermediate Filament Proteins / metabolism
Mice, Transgenic
Nerve Tissue Proteins*
Neural Cell Adhesion Molecule L1*
Neural Cell Adhesion Molecules / genetics,  metabolism*
Neurons / cytology,  metabolism,  transplantation*
Oligodendroglia / cytology,  metabolism
Sialic Acids / genetics,  metabolism*
Stem Cell Transplantation*
Stem Cells / cytology,  metabolism
Thalamus / cytology,  growth & development,  surgery
Reg. No./Substance:
0/Antigens, Differentiation; 0/Culture Media; 0/Gangliosides; 0/Glial Fibrillary Acidic Protein; 0/Intermediate Filament Proteins; 0/Nerve Tissue Proteins; 0/Neural Cell Adhesion Molecule L1; 0/Neural Cell Adhesion Molecules; 0/Sialic Acids; 0/nestin; 0/oligodendrocyte O antigen, mouse; 0/polysialyl neural cell adhesion molecule; 62010-37-1/ganglioside, GD3

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