| Migration of melanoblasts into the developing murine hair follicle is accompanied by transient c-Kit expression. | |
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MedLine Citation:
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PMID: 12019292 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Disruption of the c-Kit/stem cell factor (SCF) signaling pathway interferes with the survival, migration, and differentiation of melanocytes during generation of the hair follicle pigmentary unit. We examined c-Kit, SCF, and S100 (a marker for precursor melanocytic cells) expression, as well as melanoblast/melanocyte ultrastructure, in perinatal C57BL/6 mouse skin. Before the onset of hair bulb melanogenesis (i.e., stages 0-4 of hair follicle morphogenesis), strong c-Kit immunoreactivity (IR) was seen in selected non-melanogenic cells in the developing hair placode and hair plug. Many of these cells were S100-IR and were ultrastructurally identified as melanoblasts with migratory appearance. During the subsequent stages (5 and 6), increasingly dendritic c-Kit-IR cells successively invaded the hair bulb, while S100-IR gradually disappeared from these cells. Towards the completion of hair follicle morphogenesis (stages 7 and 8), several distinct follicular melanocytic cell populations could be defined and consisted broadly of (a) undifferentiated, non-pigmented c-Kit-negative melanoblasts in the outer root sheath and bulge and (b) highly differentiated melanocytes adjacent to the hair follicle dermal papilla above Auber's line. Widespread epithelial SCF-IR was seen throughout hair follicle morphogenesis. These findings suggest that melanoblasts express c-Kit as a prerequisite for migration into the SCF-supplying hair follicle epithelium. In addition, differentiated c-Kit-IR melanocytes target the bulb, while non-c-Kit-IR melanoblasts invade the outer root sheath and bulge in fully developed hair follicles. |
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Authors:
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Eva M J Peters; Desmond J Tobin; Natasha Botchkareva; Marcus Maurer; Ralf Paus |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society Volume: 50 ISSN: 0022-1554 ISO Abbreviation: J. Histochem. Cytochem. Publication Date: 2002 Jun |
Date Detail:
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Created Date: 2002-05-20 Completed Date: 2002-06-11 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9815334 Medline TA: J Histochem Cytochem Country: United States |
Other Details:
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Languages: eng Pagination: 751-66 Citation Subset: IM |
Affiliation:
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Department of Dermatology, University Hospital Eppendorf, University of Hamburg, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Animals, Newborn Cell Movement Hair Follicle / embryology, physiology*, ultrastructure Immunohistochemistry Melanocytes / metabolism, physiology*, ultrastructure Mice Mice, Inbred C57BL Microscopy, Electron Morphogenesis Stem Cell Factor / metabolism* Stem Cells / metabolism, physiology, ultrastructure |
| Chemical | |
Reg. No./Substance:
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0/Stem Cell Factor |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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