Document Detail

Migration and differentiation of neural cell lines transplanted into mouse brains.
MedLine Citation:
PMID:  17651850     Owner:  NLM     Status:  MEDLINE    
In the past few years, the plasticity of the regional specification of the CNS has been widely debated on the results from in utero transplantation. Two different results are reported with this transplantation method. One is that the distribution of transplanted cells is dependent on the donor origin, and the other is that the distribution is independent on the donor cell origin. The present study attempted to examine closely the plasticity of the regional specification by in utero transplantation method with clonal neural cell lines, 2Y-3t and 2Y-5o2b. These lines were established from a cerebellum of an adult p53-deficient mouse. Our results showed that transplanted cells migrated into various regions of the CNS and supported the independent distribution. Moreover, different distribution patterns of transplanted cells were observed between host sexes. Labeled cells were localized around the ventricle of neonatal host brains, where they were undifferentiated. In 2-3 weeks after birth, labeled cells were found in the brain parenchyma and some of them took neuronal morphology. In the rostral migratory stream (RMS), cells with unipolar or bipolar morphology were still undifferentiated. In other regions, labeled cells were often associated with blood vessels; the soma were on the surface of vessels, extending processes or neurites into surrounding brain parenchyma. Time-lapse imaging demonstrated that they were migrating with blood vessels.
Shinya Honda; Kotaro Toda; Yusuke Tozuka; Syohei Yasuzawa; Kaoru Iwabuchi; Yasuhiro Tomooka
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-06-20
Journal Detail:
Title:  Neuroscience research     Volume:  59     ISSN:  0168-0102     ISO Abbreviation:  Neurosci. Res.     Publication Date:  2007 Oct 
Date Detail:
Created Date:  2007-09-25     Completed Date:  2007-12-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8500749     Medline TA:  Neurosci Res     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  124-35     Citation Subset:  IM    
Department of Biological Science and Technology, and Tissue Engineering Research Center, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan.
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MeSH Terms
Animals, Newborn
Biological Markers / metabolism
Blood Vessels / embryology
Brain / embryology*,  physiology,  surgery
Brain Tissue Transplantation / methods*
Cell Differentiation / physiology*
Cell Line
Cell Lineage / physiology
Cell Movement / physiology*
Cell Shape / physiology
Cell Survival / physiology
Clone Cells / cytology,  metabolism,  transplantation
Graft Survival / physiology*
Mice, Knockout
Nerve Tissue Proteins / metabolism
Neurites / ultrastructure
Neurons / cytology,  metabolism,  transplantation*
Organ Culture Techniques
Sex Characteristics
Time Factors
Tumor Suppressor Protein p53 / genetics
Reg. No./Substance:
0/Biological Markers; 0/Nerve Tissue Proteins; 0/Tumor Suppressor Protein p53

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