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Migration of bone marrow-derived cells and improved perfusion after treatment with Erythropoietin in a murine model of myocardial infarction.
MedLine Citation:
PMID:  21362129     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Objectives: Erythropoietin (EPO) was shown to have protective effects after myocardial infarction (MI) by neovascularization and antiapoptotic mechanisms. Beside direct receptor dependent mechanisms, mobilization and homing of bone marrow-derived cells (BMCs) may play a pivotal role in this regard. In the present study, we intended to track different subpopulations of BMCs and to assess serially myocardial perfusion changes in EPO-treated mice after MI. Methods. To allow tracking of BMCs, we used a chimeric mouse model. Therefore, mice (C57BL/6J) were sublethally irradiated, and bone marrow (BM) from green fluorescent protein transgenic mice was transplanted. 10 weeks later coronary artery ligation was performed to induce MI. EPO was injected for 3 days with a total dose of 5000 IU/kg. Subpopulations (CD31, c-kit, CXCR-4, and Sca-1) of EGFP(+) cells were studied in peripheral blood, bone marrow and hearts by flow cytometry. Myocardial Perfusion was serially investigated in vivo by pinhole single-photon emission computed tomography (SPECT) at days 6 and 30 after MI. Results. EPO treated animals revealed an enhanced mobilization of BMCs into peripheral blood. The numbers of these cells in BM remained unchanged. Homing of all BMCs subpopulations to the ischemic myocardium was significantly increased in EPO-treated mice. Among the investigated subpopulations, EPO predominantly affected migration of CXCR-4(+) (4.3-fold increase). Repetitively SPECT analyses revealed a reduction of perfusion defects after EPO treatment over time. Conclusion. Our study shows that EPO treatment after MI enhances the migration capacity of BMCs into ischemic tissue, which may attribute to an improved perfusion and reduced size of infarction respectively.
Authors:
Stefan Brunner; Bruno C Huber; Tobias Weinberger; Marcus Vallaster; Tim Wollenweber; Armin Gerbitz; Marcus Hacker; Wolfgang-Michael Franz
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-3-1
Journal Detail:
Title:  Journal of cellular and molecular medicine     Volume:  -     ISSN:  1582-4934     ISO Abbreviation:  -     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-3-2     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101083777     Medline TA:  J Cell Mol Med     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
2011.
Affiliation:
Klinikum der Universität München, Medical Department I, Munich, Germany Klinikum der Universität München, Department of Nuclear Medicine, Munich, Germany Friedrich-Alexander-University, Department of Internal Medicine V, Erlangen, Germany.
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