Document Detail


Migration of Salmonella typhimurium --harboring bone marrow--derived dendritic cells towards the chemokines CCL19 and CCL21.
MedLine Citation:
PMID:  12071677     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Macrophages are considered as main cellular target encountered by the facultative intracellular bacterium Salmonella typhimurium. However, in orally infected mice these pathogens are first internalized by dendritic cells (DCs) that are located in the subepithelial dome of Peyer's patches. Moreover, DCs can penetrate the intestinal epithelium to sample bacteria. Here, we examined the interaction of Salmonella with bone marrow-derived DCs (BM-DCs). In order to study the role of DCs as vehicles for the dissemination of Salmonella, an in vitro model was established. In this model, Salmonella -activated BM-DCs enhanced surface expression of MHC class II and co-stimulatory molecules. We found that, upon maturation, BM-DCs upregulated chemokine receptor 7 (CCR7) mRNA and surface molecule expression. Salmonella -exposed DCs as well as mature DCs, but not immature DCs, were recruited towards the CC chemokines CCL19 and CCL21, two ligands of CCR7. The maturation process of DCs did neither require bacterial internalization nor viability. About one third of the migrated BM-DCs harbored intracellular bacteria, whereas the remaining two third did not contain bacteria. Salmonella, but not an apathogenic E. coli laboratory strain was capable to survive within BM-DCs. Taken together, our data implicate that DCs are first activated and subsequently utilized as carriers by Salmonella.
Authors:
Cédric Cheminay; Matthias Schoen; Michael Hensel; Alexandra Wandersee-Steinhäuser; Uwe Ritter; Heinrich Körner; Martin Röllinghoff; Joachim Hein
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Microbial pathogenesis     Volume:  32     ISSN:  0882-4010     ISO Abbreviation:  Microb. Pathog.     Publication Date:  2002 May 
Date Detail:
Created Date:  2002-06-19     Completed Date:  2002-08-07     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8606191     Medline TA:  Microb Pathog     Country:  England    
Other Details:
Languages:  eng     Pagination:  207-18     Citation Subset:  IM    
Copyright Information:
Copyright 2002 Published by Elsevier Science Ltd.
Affiliation:
Institut für Klinische Mikrobiologie, Immunologie und Hygiene, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bone Marrow / immunology,  microbiology*
Chemokine CCL19
Chemokine CCL21
Chemokines, CC / immunology*
Chemotaxis
Dendritic Cells / immunology*,  microbiology
Female
Flow Cytometry
Genes, MHC Class II / immunology
Mice
Mice, Inbred BALB C
RNA / chemistry,  genetics
Receptors, CCR7
Receptors, Chemokine / biosynthesis,  genetics,  immunology
Reverse Transcriptase Polymerase Chain Reaction
Salmonella Infections / immunology*,  microbiology
Salmonella typhimurium / growth & development,  immunology*
Chemical
Reg. No./Substance:
0/Ccl19 protein, mouse; 0/Ccl21c protein, mouse; 0/Ccr7 protein, mouse; 0/Chemokine CCL19; 0/Chemokine CCL21; 0/Chemokines, CC; 0/Receptors, CCR7; 0/Receptors, Chemokine; 63231-63-0/RNA

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