| Mifepristone-induced nitric oxide release and expression of nitric oxide synthases in the human cervix during early pregnancy. | |
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MedLine Citation:
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PMID: 16684839 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Nitric oxide (NO) is a factor in cervical ripening, perhaps under the control of progesterone. We studied the effects of the antiprogesterone mifepristone on the release of NO and on the expression of inducible NO synthase (iNOS) and endothelial NO synthase (eNOS) in the uterine cervix of women in early pregnancy. METHODS: Thirteen women were treated with oral mifepristone (200 mg), and 15 women were studied as controls. Cervical fluid samples were collected before treatment then hourly up to 3 h, and the samples were assayed for the concentration of nitric oxide metabolites (NOx). In addition, cervical biopsy samples from six women treated with mifepristone and from six controls were assessed for iNOS and eNOS by immunohistochemistry and Western blotting. RESULTS: In 1-3 h, mifepristone induced 7.4- to 17.2-fold elevations in cervical fluid NOx concentrations; no change was seen in the controls. The expression of both iNOS and eNOS was detected in the cervical cells. The expression of cervical iNOS was strong in five of the six women treated with mifepristone but was not strong in any of the six control women. CONCLUSION: This is the first study to show that mifepristone stimulates the release of NO and the expression of iNOS in cervical cells of women in early pregnancy. This may be one mechanism by which mifepristone initiates cervical ripening. |
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Authors:
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Mervi Väisänen-Tommiska; Ralf Butzow; Olavi Ylikorkala; Tomi S Mikkola |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-05-09 |
Journal Detail:
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Title: Human reproduction (Oxford, England) Volume: 21 ISSN: 0268-1161 ISO Abbreviation: Hum. Reprod. Publication Date: 2006 Aug |
Date Detail:
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Created Date: 2006-08-04 Completed Date: 2006-09-25 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8701199 Medline TA: Hum Reprod Country: England |
Other Details:
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Languages: eng Pagination: 2180-4 Citation Subset: IM |
Affiliation:
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Department of Obstetrics and Gynecology, Helsinki University Central Hospital, Finland. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Abortion, Induced Adult Cervix Uteri / drug effects, enzymology, physiology* Female Humans Immunohistochemistry Mifepristone / pharmacology* Nitric Oxide / secretion* Nitric Oxide Synthase Type II / biosynthesis* Nitric Oxide Synthase Type III / biosynthesis* Pregnancy Pregnancy Trimester, First* |
| Chemical | |
Reg. No./Substance:
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10102-43-9/Nitric Oxide; 84371-65-3/Mifepristone; EC 1.14.13.39/NOS3 protein, human; EC 1.14.13.39/Nitric Oxide Synthase Type II; EC 1.14.13.39/Nitric Oxide Synthase Type III |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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