Document Detail

Midkine accumulated in nucleolus of HepG2 cells involved in rRNA transcription.
MedLine Citation:
PMID:  18985819     Owner:  NLM     Status:  MEDLINE    
AIM: To investigate the ultrastructural location of midkine (MK) in nucleolus and function corresponding to its location. METHODS: To investigate the ultrastructural location of MK in nucleolus with immunoelectronic microscopy. To study the role that MK plays in ribosomal biogenesis by real-time PCR. The effect of MK on anti-apoptotic activity of HepG2 cells was studied with FITC-conjugated annexin V and propidium iodide PI double staining through FACS assay. RESULTS: MK mainly localized in the granular component (GC), dense fibrillar component (DFC) and the border between the DFC and fibrillar center (FC). The production of 45S precursor rRNA level was decreased significantly in the presence of MK antisense oligonucleotide in the HepG2 cells. Furthermore, it was found that exogenous MK could protect HepG2 from apoptosis significantly. CONCLUSION: MK was constitutively translocated to the nucleolus of HepG2 cells, where it accumulated and mostly distributed at DFC, GC components and at the region between FC and DFC, MK played an important role in rRNA transcription, ribosome biogenesis, and cell proliferation in HepG2 cells. MK might serve as a molecular target for therapeutic intervention of human carcinomas.
Li-Cheng Dai; Jian-Zhong Shao; Li-Shan Min; Yong-Tao Xiao; Li-Xin Xiang; Zhi-Hong Ma
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  World journal of gastroenterology : WJG     Volume:  14     ISSN:  1007-9327     ISO Abbreviation:  World J. Gastroenterol.     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-11-05     Completed Date:  2009-04-02     Revised Date:  2010-09-22    
Medline Journal Info:
Nlm Unique ID:  100883448     Medline TA:  World J Gastroenterol     Country:  China    
Other Details:
Languages:  eng     Pagination:  6249-53     Citation Subset:  IM    
Huzhou key laboratory of Molecular Medicine, Huzhou Central Hospital, Huzhou 313000, China.
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MeSH Terms
Active Transport, Cell Nucleus
Carcinoma, Hepatocellular / genetics,  metabolism*,  ultrastructure
Cell Line, Tumor
Cell Nucleolus / metabolism*,  ultrastructure
Cell Proliferation
Cell Separation
Flow Cytometry
Liver Neoplasms / genetics,  metabolism*,  ultrastructure
Microscopy, Immunoelectron
Nerve Growth Factors / genetics,  metabolism*
Oligonucleotides, Antisense / metabolism
Polymerase Chain Reaction
RNA, Ribosomal / metabolism*
Transcription, Genetic*
Reg. No./Substance:
0/MDK protein, human; 0/Nerve Growth Factors; 0/Oligonucleotides, Antisense; 0/RNA, Ribosomal; 0/RNA, ribosomal, 45S

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