Document Detail


Midbrain dopaminergic cell loss in Parkinson's disease and MPTP-induced parkinsonism: sparing of calbindin-D28k-containing cells.
MedLine Citation:
PMID:  1353337     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Computer imaging and immunohistochemical staining techniques were used to determine which midbrain dopaminergic (DA) cells are spared in Parkinson's disease (PD), and in animals treated with the DA neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), and whether the spared cells contain the calcium-binding protein, calbindin-D28k (CaBP). The PD patients had more than 55% fewer midbrain DA neurons than age-matched normal subjects. The cell loss occurred within the combined substantia nigra and retrorubral area (greater than 61%; DA nuclei A9 and A8, respectively), and the ventral tegmental area (greater than 42%; DA nucleus A10). The cell loss was greatest within the ventral portion of the nucleus A9. A similar pattern of DA cell loss was observed in MPTP-treated Macaca fascicularis monkeys. The CaBP-containing cells were located specifically in the cell regions spared by PD and by MPTP-treatment in both monkeys and C57BL/6 mice. These data suggest that PD and MPTP both destroy the same population of midbrain DA neurons within nuclei A8, A9, and A10, and that perhaps CaBP protects the DA neurons from cell death caused by both PD and MPTP.
Authors:
D C German; K F Manaye; P K Sonsalla; B A Brooks
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Annals of the New York Academy of Sciences     Volume:  648     ISSN:  0077-8923     ISO Abbreviation:  Ann. N. Y. Acad. Sci.     Publication Date:  1992 May 
Date Detail:
Created Date:  1992-08-21     Completed Date:  1992-08-21     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7506858     Medline TA:  Ann N Y Acad Sci     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  42-62     Citation Subset:  IM    
Affiliation:
Department of Psychiatry, University of Texas Southwestern Medical Center, Dallas 75235.
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MeSH Terms
Descriptor/Qualifier:
Aged
Animals
Calcium-Binding Protein, Vitamin D-Dependent / metabolism*
Cell Death
Dopamine / metabolism*
Female
Humans
MPTP Poisoning*
Macaca fascicularis
Male
Mesencephalon / drug effects,  metabolism*,  pathology
Middle Aged
Parkinson Disease / metabolism*,  pathology
Parkinson Disease, Secondary / chemically induced,  metabolism*,  pathology
Tyrosine 3-Monooxygenase / analysis,  metabolism
Grant Support
ID/Acronym/Agency:
AG-08013/AG/NIA NIH HHS; NS-22187/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Calcium-Binding Protein, Vitamin D-Dependent; 0/calbindin; EC 1.14.16.2/Tyrosine 3-Monooxygenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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