Document Detail


Microwave fixation of cells in tissue culture.
MedLine Citation:
PMID:  6105733     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Use of microwave irradiation has been evaluated for the in situ fixation of cells grown in tissue culture prior to fluorescent antibody staining. The results show total retention of cell protein in the matrix with microwave fixation, whereas 40-50% of the protein is lost during conventional formaldehyde fixation. Fluorescent antibody staining of cells shows that this protein loss reflects cell loss. A further advantage of microwave fixation is that photometric quantitation of fluorescence appears possible by random scanning of fluorescent antibody stained populations.
Authors:
M K Patterson; R Bulard
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Stain technology     Volume:  55     ISSN:  0038-9153     ISO Abbreviation:  Stain Technol     Publication Date:  1980 Mar 
Date Detail:
Created Date:  1980-10-27     Completed Date:  1980-10-27     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0404535     Medline TA:  Stain Technol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  71-5     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Cell Adhesion
Cell Line
Cytological Techniques*
Fibronectins / analysis
Fluorescent Antibody Technique
Humans
Lung
Microwaves*
gamma-Glutamyltransferase / analysis
Chemical
Reg. No./Substance:
0/Fibronectins; EC 2.3.2.2/gamma-Glutamyltransferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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