Document Detail


Microwave ablation of ex vivo human liver and colorectal liver metastases with a novel 14.5 GHz generator.
MedLine Citation:
PMID:  22235784     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Purpose: This study assessed the relationship between time, power and ablation size using a novel high-frequency 14.5 GHz microwave applicator in ex vivo human hepatic parenchyma and colorectal liver metastases. Previous examination has demonstrated structurally normal but non-viable cells within the ablation zone. This study aimed to further investigate how ablation affects these cells, and to confirm non-viability. Materials and methods: Ablations were performed in ex vivo human hepatic parenchyma and tumour for a variety of time (10-180 s) and power (10-50 W) settings. Histological examination was performed to assess cellular anatomy, whilst enzyme histochemistry was used to confirm cellular non-viability. Transmission electron microscopy was used to investigate the subcellular structural effects of ablation within these fixed cells. Preliminary proteomic analysis was also performed to explore the mechanism of microwave cell death. Results: Increasing time and power settings led to a predictable and reproducible increase in size of ablation. At 50 W and 180 s application, a maximum ablation diameter of 38.8 mm (±1.3) was produced. Ablations were produced rapidly, and at all time and power settings ablations remained spherical (longest:shortest diameter <1.2). Routine histological analysis using haematoxylin-eosin (H&E) confirmed well preserved cellular anatomy despite ablation. Transmission electron microscopy demonstrated marked subcellular damage. Enzyme histochemistry showed complete absence of viability in ablated tissue. Conclusions: Large spherical ablation zones can be rapidly and reproducibly achieved in ex vivo human hepatic parenchyma and colorectal liver metastases using a 14.5 GHz microwave generator. Despite well preserved cellular appearance, ablated tissue is non-viable.
Authors:
Robert P Jones; Neil R Kitteringham; Monica Terlizzo; Christopher Hancock; Declan Dunne; Stephen W Fenwick; Graeme J Poston; Paula Ghaneh; Hassan Z Malik
Related Documents :
8661514 - Swelling and ca2+-activated anion conductances in c127 epithelial cells expressing wt a...
10833494 - Cystic fibrosis transmembrane conductance regulator currents in guinea pig pancreatic d...
17389364 - The role of pannexin 1 hemichannels in atp release and cell-cell communication in mouse...
19721284 - Effect of surface roughness on initial responses of osteoblast-like cells on two types ...
20188954 - Fluorescent quantum dot-labeled aptamer bioprobes specifically targeting mouse liver ca...
15633804 - Antioxidant activity of porcelainberry (ampelopsis brevipedunculata (maxim.) trautv.).
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  International journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group     Volume:  28     ISSN:  1464-5157     ISO Abbreviation:  Int J Hyperthermia     Publication Date:  2012  
Date Detail:
Created Date:  2012-01-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8508395     Medline TA:  Int J Hyperthermia     Country:  England    
Other Details:
Languages:  eng     Pagination:  43-54     Citation Subset:  IM    
Affiliation:
Centre for Drug Safety Science, Department of Pharmacology, University of Liverpool .
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Modified vaccinia virus Ankara delivers a robust surrogate marker for immune monitoring to sarcoma c...
Next Document:  CT-based temperature monitoring during hepatic RF ablation: Feasibility in an animal model.