Document Detail


Microwave irradiation decreases ATP, increases free [Mg²⁺], and alters in vivo intracellular reactions in rat brain.
MedLine Citation:
PMID:  23013291     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Rapid inactivation of metabolism is essential for accurately determining the concentrations of metabolic intermediates in the in vivo state. We compared a broad spectrum of energetic intermediate metabolites and neurotransmitters in brains obtained by microwave irradiation to those obtained by freeze blowing, the most rapid method of extracting and freezing rat brain. The concentrations of many intermediates, cytosolic free NAD(P)(+) /NAD(P)H ratios, as well as neurotransmitters were not affected by the microwave procedure. However, the brain concentrations of ATP were about 30% lower, whereas those of ADP, AMP, and GDP were higher in the microwave-irradiated compared with the freeze-blown brains. In addition, the hydrolysis of approximately 1 μmol/g of ATP, a major in vivo Mg(2+) -binding site, was related to approximately five-fold increase in free [Mg(2+) ] (0.53 ± 0.07 mM in freeze blown vs. 2.91 mM ± 0.48 mM in microwaved brains), as determined from the ratio [citrate]/[isocitrate]. Consequently, many intracellular properties, such as the phosphorylation potential and the ∆G' of ATP hydrolysis were significantly altered in microwaved tissue. The determinations of some glycolytic and TCA cycle metabolites, the phosphorylation potential, and the ∆G' of ATP hydrolysis do not represent the in vivo state when using microwave-fixed brain tissue.
Authors:
Shireesh Srivastava; Yoshihiro Kashiwaya; Xuesong Chen; Jonathan D Geiger; Robert Pawlosky; Richard L Veech
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Journal of neurochemistry     Volume:  123     ISSN:  1471-4159     ISO Abbreviation:  J. Neurochem.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-11-08     Completed Date:  2013-01-10     Revised Date:  2013-12-04    
Medline Journal Info:
Nlm Unique ID:  2985190R     Medline TA:  J Neurochem     Country:  England    
Other Details:
Languages:  eng     Pagination:  668-75     Citation Subset:  IM    
Copyright Information:
Published 2012. This article is a US Government work and is in the public domain in the USA.
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphate / analysis*,  metabolism,  radiation effects
Animals
Brain / metabolism,  radiation effects*
Brain Chemistry / radiation effects*
Electrophoresis, Capillary
Freezing
Gas Chromatography-Mass Spectrometry
Intracellular Space / chemistry,  radiation effects*
Magnesium / analysis*
Male
Microwaves
Rats
Rats, Sprague-Dawley
Tissue Fixation / methods*
Grant Support
ID/Acronym/Agency:
2P20RR017699/RR/NCRR NIH HHS; R01 NS065957/NS/NINDS NIH HHS; R01NS065957/NS/NINDS NIH HHS; Z01 AA000110-10/AA/NIAAA NIH HHS
Chemical
Reg. No./Substance:
8L70Q75FXE/Adenosine Triphosphate; I38ZP9992A/Magnesium
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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