| Microviscosity of amniotic fluid phospholipids, and its importance in determining fetal lung maturity. | |
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MedLine Citation:
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PMID: 761383 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Fluorescence polarization measurements of microviscosity (apparent viscosity within the hydrophobic center of lipid bilayers) of amniotic fluid correlate well with lecithin/sphingomyelin ratios determined by thin-layer chromatography. In addition to lecithin, phosphatidylglycerol and phosphatidylinositol are important for determining fetal lung maturity, but the lecithin/sphingomyelin ratio gives no information concerning these other phospholipids. The microviscosity of sphingomyelin significantly exceeded that of lecithin over the temperature range 25--37 degrees C; values for phosphatidylglycerol, phosphatidylinositol, and phosphatidylserine were lower. Phosphatidylglycerol, phosphatidylinositol, and phosphatidylserine, added individually, significantly decreased the microviscosity of dispersions with lecithin/sphingomyelin ratios corresponding either to fetal lung immaturity or maturity. Phosphatidylglycerol caused the greatest decrease in both. Mixtures of the three phospholipids in the proportions found in term amniotic fluid decreased the microviscosity of fluids with either mature or immature lecithin/sphingomyelin ratios by 23--27%. When each was present in the proportion found in tracheal aspirate (twice that of term amniotic fluid), the decreases uere 46--50%. This technique quickly and precisely indicates not only fetal lung maturity but also the presence of important phospholipids other than lecithin and sphingomyelin. |
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Authors:
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T A Blumenfeld; H S Cheskin; M Shinitzky |
Related Documents
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6933813 - Phospholipid studies of the amniotic fluid in diabetic pregnancy. 216713 - Enamel matrix proteins in normal and abnormal amelogenesis. 7949823 - Thrombocytopenia in pregnancy. |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Clinical chemistry Volume: 25 ISSN: 0009-9147 ISO Abbreviation: Clin. Chem. Publication Date: 1979 Jan |
Date Detail:
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Created Date: 1979-04-25 Completed Date: 1979-04-25 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 9421549 Medline TA: Clin Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 64-7 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amniotic Fluid
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analysis* Female Fetus Humans Lung / embryology* Phospholipids / analysis* Pregnancy Temperature Viscosity |
| Chemical | |
Reg. No./Substance:
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0/Phospholipids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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