Document Detail


Microvessels of the chick chorioallantoic membrane uniformly restrict albumin extravasation during angiogenesis and endothelial cytodifferentiation.
MedLine Citation:
PMID:  9225483     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The present study served to evaluate extravasation of albumin across the microvascular endothelium of the chick chorioallantoic membrane (CAM) during the establishment of endothelial barrier functions (days 4.5 to 6.0), during a rapid phase of normigenesis (day 10), and after initiation of endothelial cytodifferentiation (day 14). CAM preparations in shell-less cultures were evaluated by intravital fluorescence confocal microscopy and videodensitometry after microinjections of chicken serum albumin (CSA) conjugated to Fluorescein isothiocyanate (FITC) (1% in Avian Ringers). At each observed temporal stage, FITC-CSA extravasation from the CAM pre-capillaries, capillaries, and post-capillaries was uniformly negligible. Since the endothelial glycocalyx imparts a net negative luminal charge, impedance of CSA transport by mutual charge repulsion was also evaluated. Accordingly, CAM microvessels were pre-infused with protamine sulfate (100 microliters/ml) to neutralize the negative charge. However, interstitial accumulation of FITC-CSA remained negligible. Thus, the presence of tight junctional clefts and paucity of plasmalemmal vesicles during CAM normigenesis probably serve as the principal morphologic correlates to the observed albumin restriction. Further, the normigenic CAM endothelium presents a more restrictive barrier to macromolecules than that of most angiogenic endothelia.
Authors:
A Cruz; V Rizzo; D O De Fouw
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Tissue & cell     Volume:  29     ISSN:  0040-8166     ISO Abbreviation:  Tissue Cell     Publication Date:  1997 Jun 
Date Detail:
Created Date:  1997-08-19     Completed Date:  1997-08-19     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0214745     Medline TA:  Tissue Cell     Country:  SCOTLAND    
Other Details:
Languages:  eng     Pagination:  277-81     Citation Subset:  IM    
Affiliation:
Department of Anatomy, Cell Biology and injury Sciences, UMDN-J-New Jarsey Medical School, Newark 07103, USA.
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MeSH Terms
Descriptor/Qualifier:
Allantois / blood supply
Animals
Capillaries / embryology*,  physiology
Capillary Permeability*
Cell Differentiation
Chick Embryo
Chorion / blood supply
Endothelium, Vascular / cytology*,  metabolism
Fluorescein-5-isothiocyanate / metabolism
Image Processing, Computer-Assisted
Neovascularization, Physiologic*
Serum Albumin / metabolism*
Grant Support
ID/Acronym/Agency:
GM17238/GM/NIGMS NIH HHS; HL 47936/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Serum Albumin; 3326-32-7/Fluorescein-5-isothiocyanate

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