Document Detail


Microvesicles are a source of contaminating cellular proteins found in purified HIV-1 preparations.
MedLine Citation:
PMID:  9126269     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Identification and quantitation of cellular proteins associated with HIV-1 particles are complicated by the presence of nonvirion-associated cellular proteins that copurify with virions. Many cellular proteins are associated with nonviral particles that bud from the surface of cells called microvesicles. Microvesicles band in sucrose gradients in a range of densities that includes the same density as retroviruses. To characterize these microvesicles, HIV-1-infected and uninfected human T-cell lines were propagated and virus and microvesicles were purified from clarified cell culture supernatants by sucrose density gradient centrifugation or centrifugation through 20% sucrose pads. Microvesicles were found to contain various proteins, including HLA DR and beta 2-M, and a substantial amount of RNA and DNA. The concentrations of HIV-1 p24CA, HLA DR and beta 2-microglobulin (beta 2-M) were determined by radioimmunoassay. The ratios of HIV-1 p24CA to HLA DR and beta 2-M were found to vary with respect to the HIV-1 isolate, host cell, and other factors. Electron microscopic analysis of microvesicles revealed that they consisted of particles of various sizes and morphologies. Although HIV-1 particles are known to contain some cellular proteins, microvesicles from HIV-1 infected H9 cells appeared to contain little or no HIV-1 gp120SU.
Authors:
J W Bess; R J Gorelick; W J Bosche; L E Henderson; L O Arthur
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Virology     Volume:  230     ISSN:  0042-6822     ISO Abbreviation:  Virology     Publication Date:  1997 Mar 
Date Detail:
Created Date:  1997-06-26     Completed Date:  1997-06-26     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0110674     Medline TA:  Virology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  134-44     Citation Subset:  IM; X    
Affiliation:
AIDS Vaccine Program, SAIC, National Cancer Institute-Frederick Cancer Research and Development Center, Maryland 21702-1201, USA.
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MeSH Terms
Descriptor/Qualifier:
HIV Core Protein p24 / analysis
HIV Envelope Protein gp120 / analysis
HIV-1 / isolation & purification*
HLA-DR Antigens / analysis
Humans
Leukocytes, Mononuclear / virology
Organelles / chemistry
Proteins / analysis*
T-Lymphocytes / chemistry,  virology
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/HIV Core Protein p24; 0/HIV Envelope Protein gp120; 0/HLA-DR Antigens; 0/Proteins

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