| Microvesicles are a source of contaminating cellular proteins found in purified HIV-1 preparations. | |
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MedLine Citation:
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PMID: 9126269 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Identification and quantitation of cellular proteins associated with HIV-1 particles are complicated by the presence of nonvirion-associated cellular proteins that copurify with virions. Many cellular proteins are associated with nonviral particles that bud from the surface of cells called microvesicles. Microvesicles band in sucrose gradients in a range of densities that includes the same density as retroviruses. To characterize these microvesicles, HIV-1-infected and uninfected human T-cell lines were propagated and virus and microvesicles were purified from clarified cell culture supernatants by sucrose density gradient centrifugation or centrifugation through 20% sucrose pads. Microvesicles were found to contain various proteins, including HLA DR and beta 2-M, and a substantial amount of RNA and DNA. The concentrations of HIV-1 p24CA, HLA DR and beta 2-microglobulin (beta 2-M) were determined by radioimmunoassay. The ratios of HIV-1 p24CA to HLA DR and beta 2-M were found to vary with respect to the HIV-1 isolate, host cell, and other factors. Electron microscopic analysis of microvesicles revealed that they consisted of particles of various sizes and morphologies. Although HIV-1 particles are known to contain some cellular proteins, microvesicles from HIV-1 infected H9 cells appeared to contain little or no HIV-1 gp120SU. |
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Authors:
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J W Bess; R J Gorelick; W J Bosche; L E Henderson; L O Arthur |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Virology Volume: 230 ISSN: 0042-6822 ISO Abbreviation: Virology Publication Date: 1997 Mar |
Date Detail:
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Created Date: 1997-06-26 Completed Date: 1997-06-26 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0110674 Medline TA: Virology Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 134-44 Citation Subset: IM; X |
Affiliation:
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AIDS Vaccine Program, SAIC, National Cancer Institute-Frederick Cancer Research and Development Center, Maryland 21702-1201, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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HIV Core Protein p24
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analysis HIV Envelope Protein gp120 / analysis HIV-1 / isolation & purification* HLA-DR Antigens / analysis Humans Leukocytes, Mononuclear / virology Organelles / chemistry Proteins / analysis* T-Lymphocytes / chemistry, virology Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/HIV Core Protein p24; 0/HIV Envelope Protein gp120; 0/HLA-DR Antigens; 0/Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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