Document Detail

Microvascular preconditioning is not detectable by corrosion casting in the isolated perfused rat heart after 30 minutes of ischaemia.
MedLine Citation:
PMID:  18273729     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: Ischaemic preconditioning protects the myocardium from ischaemic injury and may also protect the vascular endothelium from the deleterious effects of ischaemia and reperfusion. We examined the possibility that ischaemic preconditioning might preserve the integrity of the coronary microcirculation following ischaemia and reperfusion. METHODS: Isolated rat hearts were perfused in Langendorff mode for 30 minutes and then subjected to 30 minutes of global ischaemia with or without ischaemic preconditioning (threexthree minute cycles). Some hearts underwent an additional 60 minutes of reperfusion. At the end of each protocol, microvascular corrosion casts were made by methylmethacrylate injection. RESULTS: Median left ventricular capillary density [interquartile range] after ischaemia was slightly but not significantly better with preconditioning at 6.8 [4.0-14.7]x10(-2) vs. 5.2 [2.6-7.1]x10(-2) (p=0.13). After 60 min of reperfusion, capillary density in preconditioned left ventricles was 20.7 [10.7-22.8]x10(-2) vs. 16.0 [10.2-23.0]x10(-2) for untreated ventricles (p=0.47). Coronary blood flow and heart rate were unchanged from before ischaemia. CONCLUSIONS: Ischaemia for 30 minutes induced global left ventricular capillary loss which was unmodified by preconditioning. We did not demonstrate vascular preconditioning using this model.
A Arangannal; A McGarrity; D J Wheatley; P R Belcher
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Scandinavian cardiovascular journal : SCJ     Volume:  42     ISSN:  1401-7431     ISO Abbreviation:  Scand. Cardiovasc. J.     Publication Date:  2008 Feb 
Date Detail:
Created Date:  2008-02-14     Completed Date:  2008-07-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9708377     Medline TA:  Scand Cardiovasc J     Country:  England    
Other Details:
Languages:  eng     Pagination:  90-5     Citation Subset:  IM    
Division of Cardiovascular Sciences, University of Glasgow, Glasgow, Scotland, UK.
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MeSH Terms
Coronary Circulation
Coronary Vessels / physiopathology,  ultrastructure*
Corrosion Casting*
Disease Models, Animal
Heart Rate
Ischemic Preconditioning, Myocardial*
Microcirculation / ultrastructure
Microscopy, Electron, Scanning
Myocardial Ischemia / pathology*,  physiopathology,  therapy
Myocardial Reperfusion Injury / pathology*,  physiopathology,  prevention & control
Rats, Sprague-Dawley
Time Factors

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