| Microvascular architecture of the elastase emphysemic hamster lung. | |
| | |
MedLine Citation:
|
PMID: 1797986 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Vascular corrosion casts of normal and elastase-induced emphysemic hamster lungs, prepared with a low viscosity resin mixture consisting of Mercox and Sevriton, were observed by scanning electron microscopy. Casts were quantitated by measuring vascular volume or determining non-alveolar air space using confocal laser scanning microscopy. Normal lung casts were characterized by well-organized fields of alveoli (about 70 microns in diameter) connected by distinct alveolar ducts. Emphysemic lung casts exhibited numerous bullae (often as large as 0.5 mm in diameter). The vasculature of the bullae indicated that they were formed by destruction of alveolar walls and subsequent coalescence of numerous alveolae. Remnants of alveolar walls, consisting of shallow ridges of capillaries, lined the bases of the bullae. Vascular volumes expressed as cast volume/total tissue volume were calculated at 20% and 12% for uninflated and inflated lungs, respectively, for both control and emphysemic lungs. Four months after elastase instillation, nonalveolar air space of the emphysemic lungs was increased by 73% over controls. These observations indicate that elastase emphysema results, initially, in remodeling of the alveolar structure (bullae formation) and loss of surface area for gas exchange, rather than from extensive loss of vasculature. Vascular corrosion casting is a useful technique for monitoring emphysema both morphologically and quantitatively. |
| | |
Authors:
|
F E Hossler; J E Douglas; A Verghese; L Neal |
Related Documents
:
|
7700166 - Lung function and exercise capacity in survivors of childhood leukaemia. 3195806 - Lung function impairment as a guide to exercise limitation in work-related lung disorders. 2222016 - Tumor necrosis factor-alpha mediates acid aspiration-induced systemic organ injury. 16456146 - Lung function and exercise capacity in young adults born prematurely. 15262636 - Knee laxity does not vary with the menstrual cycle, before or after exercise. 21088206 - Influence of training and maturity status on the cardiopulmonary responses to ramp incr... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Journal of electron microscopy technique Volume: 19 ISSN: 0741-0581 ISO Abbreviation: J Electron Microsc Tech Publication Date: 1991 Dec |
Date Detail:
|
Created Date: 1992-04-15 Completed Date: 1992-04-15 Revised Date: 2005-11-17 |
Medline Journal Info:
|
Nlm Unique ID: 8502171 Medline TA: J Electron Microsc Tech Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 406-18 Citation Subset: IM |
Affiliation:
|
Department of Anatomy, James H. Quillen College of Medicine, Johnson City, Tennessee 37614. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Corrosion Casting Cricetinae Lung / blood supply* Male Mesocricetus Microcirculation Pancreatic Elastase Pulmonary Emphysema / chemically induced, pathology* |
| Chemical | |
Reg. No./Substance:
|
EC 3.4.21.36/Pancreatic Elastase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Vasculature of the fish gill: anatomical correlates of physiological functions.
Next Document: Scanning electron microscopy of the capillary loops in the dermal papillae of the hand in primates, ...