| Microvascular response to shock wave application in striated skin muscle. | |
| | |
MedLine Citation:
|
PMID: 20444475 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
BACKGROUND: This study aims to quantify by intravital microscopy the microhemodynamic response after extracorporeal shock wave application (ESWA) to the physiologic microcirculation of the mouse dorsal skinfold chamber. MATERIALS AND METHODS: ESWA was carried out using an electrohydraulic shock wave source. Two different shock wave doses of 500 and 1000 pulses at an energy flux rate of 0.08 mJ/mm(2) and a frequency of 4 Hz were compared with sham-operated animals. Microcirculatory analyses were performed at baseline (BL) and during a 3 d observation period after ESWA. The expression of caspase-3 (casp-3), proliferating cell nuclear antibody (PCNA), von Willebrand factor (vWF), and endothelial nitric oxide synthase (eNOS) were analyzed semiquantitatively by immunohistochemistry. RESULTS: ESWA provoked a significant and persistent increase of functional capillary density (FCD) throughout the observation period, reaching a maximum (140% ± 5% of BL, P < 0.05 versus sham) after 1 d when animals were treated with 1000 pulses. ESWA induced a slight increase of leukocyte rolling (∼2- to ∼3.5-fold, P < 0.05) and leukocyte adherence (∼1.5- to ∼2-fold, P < 0.05) to the endothelial lining of postcapillary venules. One day following ESWA, we observed enhanced expression of casp-3 (∼3- to ∼4-fold), PCNA (∼9- to ∼14-fold), vWF (∼11- to ∼14-fold), and eNOS (∼3-fold), all P < 0.05. CONCLUSION: This study shows that ESWA provokes a favorable persistent increase of patent capillaries, however accompanied by a transient and slight inflammatory response but also by dose-dependant apoptotic cell death. Our data suggest that ESWA might represent a noninvasive biomechanical tool to treat critically perfused and endangered tissues, but certainly warrants further investigation. |
| | |
Authors:
|
Maurizio Calcagni; Fanfan Chen; Dominik C Högger; Nicole Lindenblatt; Marius Keel; Pietro Giovanoli; Claudio Contaldo |
Related Documents
:
|
8970045 - Protective effects of monoclonal antibody to vla-4 on leukocyte adhesion and course of ... 12480815 - Estrogens inhibit angiotensin ii-induced leukocyte-endothelial cell interactions in viv... 8548905 - Nitric oxide inhibits numerous features of mast cell-induced inflammation. 9335315 - The role of shear forces in ischemia/reperfusion-induced neutrophil rolling and adhesion. 7638745 - Selective stimulation of endothelial cell proliferation with inhibition of smooth muscl... 15383545 - The fibril-associated collagen ix provides a novel mechanism for cell adhesion to carti... |
Publication Detail:
|
Type: Journal Article Date: 2010-01-05 |
Journal Detail:
|
Title: The Journal of surgical research Volume: 171 ISSN: 1095-8673 ISO Abbreviation: J. Surg. Res. Publication Date: 2011 Nov |
Date Detail:
|
Created Date: 2011-10-17 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0376340 Medline TA: J Surg Res Country: United States |
Other Details:
|
Languages: eng Pagination: 347-54 Citation Subset: IM |
Copyright Information:
|
Copyright © 2011 Elsevier Inc. All rights reserved. |
Affiliation:
|
Department of Surgery, Division of Plastic and Reconstructive Surgery, University Hospital Zurich, Zurich, Switzerland. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Newly designed flexible electrode for laparoscopic radiofrequency ablation: ex vivo and in vivo comp...
Next Document: Hemodynamic comparison of differing anastomotic geometries using magnetic resonance velocimetry.