Document Detail

Microtubules containing acetylated alpha-tubulin in mammalian cells in culture.
Jump to Full Text
MedLine Citation:
PMID:  2879846     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The subcellular distribution of microtubules containing acetylated alpha-tubulin in mammalian cells in culture was analyzed with 6-11B-1, a monoclonal antibody specific for acetylated alpha-tubulin. Cultures of 3T3, HeLa, and PtK2 cells were grown on coverslips and observed by immunofluorescence microscopy after double-staining by 6-11B-1 and B-5-1-2, a monoclonal antibody specific for all alpha-tubulins. The antibody 6-11B-1 binds to primary cilia, centrioles, mitotic spindles, midbodies, and to subsets of cytoplasmic microtubules in 3T3 and HeLa cells, but not in PtK2 cells. These observations confirm that the acetylation of alpha-tubulin is a modification occurring in different microtubule structures and in a variety of eukaryotic cells. Some features of the acetylation of cytoplasmic microtubules of mammalian cells are also described here. First, acetylated alpha-tubulin is present in microtubules that, under depolymerizing conditions, are more stable than the majority of cytoplasmic microtubules. In addition to the specific microtubule frameworks already mentioned, cytoplasmic microtubules resistant to nocodazole or colchicine, but not cold-resistant microtubules, contain more acetylated alpha-tubulin than the rest of cellular microtubules. Second, the alpha-tubulin in all cytoplasmic microtubules of 3T3 and HeLa cells becomes acetylated in the presence of taxol, a drug that stabilizes microtubules. Third, acetylation and deacetylation of cytoplasmic microtubules are reversible in cells released from exposure to 0 degrees C or antimitotic drugs. Fourth, the epitope recognized by the antibody 6-11B-1 is not absolutely necessary for cell growth and division. This epitope is absent in PtK2 cells. The acetylation of alpha-tubulin could regulate the presence of microtubules in specific intracellular spaces by selective stabilization.
Authors:
G Piperno; M LeDizet; X J Chang
Related Documents :
18787396 - Knockdown of b-raf impairs spindle formation and the mitotic checkpoint in human somati...
1558756 - Replication of basal bodies and centrioles.
9042286 - Immunoreactivity for the human hematopoietic progenitor cell antigen (cd34) in lipomato...
8056836 - Quantification of microtubule dynamics in living plant cells using fluorescence redistr...
7893706 - Relationship between methylation and acetylation of arginine-rich histones in cycling a...
12014626 - Transformation of mc3t3-e1 cells following stress and transfection with psv2neo plasmid.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of cell biology     Volume:  104     ISSN:  0021-9525     ISO Abbreviation:  J. Cell Biol.     Publication Date:  1987 Feb 
Date Detail:
Created Date:  1987-03-11     Completed Date:  1987-03-11     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0375356     Medline TA:  J Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  289-302     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Acetylation
Alkaloids / pharmacology
Animals
Cell Line
Cells, Cultured
Cold Temperature
Fluorescent Antibody Technique
Hela Cells / metabolism
Humans
Kinetics
Mice
Microtubules / drug effects,  metabolism,  ultrastructure*
Paclitaxel
Tubulin / metabolism*
Grant Support
ID/Acronym/Agency:
GM 28702/GM/NIGMS NIH HHS; S07 RR077065/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Alkaloids; 0/Tubulin; 33069-62-4/Paclitaxel
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): J Cell Biol
ISSN: 0021-9525
ISSN: 1540-8140
Publisher: The Rockefeller University Press
Article Information
Download PDF

Print publication date: Day: 1 Month: 2 Year: 1987
Volume: 104 Issue: 2
First Page: 289 Last Page: 302
ID: 2114420
Publisher Id: 87109452
PubMed Id: 2879846

Microtubules containing acetylated alpha-tubulin in mammalian cells in culture


Article Categories:
  • Articles


Previous Document:  Microtubule-acting drugs lead to the nonpolarized delivery of the influenza hemagglutinin to the cel...
Next Document:  Topography of cell wall lytic enzyme in Chlamydomonas reinhardtii: form and location of the stored e...