Document Detail


Microtubule plus-end-tracking proteins target gap junctions directly from the cell interior to adherens junctions.
MedLine Citation:
PMID:  17289573     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Gap junctions are intercellular channels that connect the cytoplasms of adjacent cells. For gap junctions to properly control organ formation and electrical synchronization in the heart and the brain, connexin-based hemichannels must be correctly targeted to cell-cell borders. While it is generally accepted that gap junctions form via lateral diffusion of hemichannels following microtubule-mediated delivery to the plasma membrane, we provide evidence for direct targeting of hemichannels to cell-cell junctions through a pathway that is dependent on microtubules; through the adherens-junction proteins N-cadherin and beta-catenin; through the microtubule plus-end-tracking protein (+TIP) EB1; and through its interacting protein p150(Glued). Based on live cell microscopy that includes fluorescence recovery after photobleaching (FRAP), total internal reflection fluorescence (TIRF), deconvolution, and siRNA knockdown, we propose that preferential tethering of microtubule plus ends at the adherens junction promotes delivery of connexin hemichannels directly to the cell-cell border. These findings support an unanticipated mechanism for protein delivery to points of cell-cell contact.
Authors:
Robin M Shaw; Alex J Fay; Manojkumar A Puthenveedu; Mark von Zastrow; Yuh-Nung Jan; Lily Y Jan
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell     Volume:  128     ISSN:  0092-8674     ISO Abbreviation:  Cell     Publication Date:  2007 Feb 
Date Detail:
Created Date:  2007-02-09     Completed Date:  2007-03-28     Revised Date:  2012-11-29    
Medline Journal Info:
Nlm Unique ID:  0413066     Medline TA:  Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  547-60     Citation Subset:  IM    
Affiliation:
Cardiovascular Research Institute, Department of Medicine, University of California, San Francisco, CA 94143, USA.
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MeSH Terms
Descriptor/Qualifier:
Adherens Junctions / metabolism*
Animals
Bacterial Proteins / genetics
Cadherins / metabolism
Connexin 43 / genetics,  metabolism
Fluorescence Recovery After Photobleaching
Gap Junctions / metabolism*
HeLa Cells
Humans
Luminescent Proteins / genetics
Microscopy, Fluorescence
Microtubule-Associated Proteins / genetics,  metabolism*
Microtubules / metabolism*
Myocytes, Cardiac / cytology
RNA Interference
RNA, Small Interfering
Rats
Rats, Sprague-Dawley
Secretory Vesicles
beta Catenin / genetics,  metabolism
Grant Support
ID/Acronym/Agency:
DA 10154/DA/NIDA NIH HHS; DA 10711/DA/NIDA NIH HHS; HL 075449/HL/NHLBI NIH HHS; K08 HL075449/HL/NHLBI NIH HHS; K08 HL075449-01A1/HL/NHLBI NIH HHS; K08 HL075449-02/HL/NHLBI NIH HHS; MH 065334/MH/NIMH NIH HHS
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/CTNNB1 protein, human; 0/Cadherins; 0/Connexin 43; 0/Luminescent Proteins; 0/MAPRE1 protein, human; 0/Microtubule-Associated Proteins; 0/RNA, Small Interfering; 0/beta Catenin; 0/yellow fluorescent protein, Bacteria; 144198-36-7/dynactin
Comments/Corrections
Erratum In:
Cell. 2008 Apr 18;133(2):376

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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