Document Detail


Microtubule distribution in dv, a maize meiotic mutant defective in the prophase to metaphase transition.
MedLine Citation:
PMID:  2407578     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Microsporogenesis in Zea mays, the meiotic reduction of diploid sporocytes to haploid microspores, proceeds through a well-defined developmental sequence. The ability to generate mutants that affect the process makes this an ideal system for elucidating the role of the cytoskeleton during plant development. We have used immunofluorescence microscopy to compare microtubule distribution in wild-type and mutant microsporocytes. During normal meiosis the distribution of microtubules follows a specific temporal and spatial pattern that reflects the polar nature of microspore formation. Perinuclear microtubule staining increases and the nucleus elongates in the future spindle axis during late prophase I. Metaphase I spindles with highly focused poles align along the long axis of the anther locule. Cytokinesis occurs perpendicular to the spindle axis. The second division axis shifts 90 degrees with respect to the first division plane, thereby yielding an isobilateral tetrad of microspores. Microtubule distribution patterns during meiosis suggest that a nuclear envelope-associated microtubule organizing center (MTOC) controls the organization of cytoplasmic microtubules and contributes to spindle formation. The meiotic mutant dv is defective in the transition from a prophase microtubule array to a metaphase spindle. Instead of converging to form focused poles, the metaphase spindle poles remain diffuse as in prometaphase. This defect correlates with several abnormalities in subsequent developmental events including the formation of multinucleate daughter cells, multiple microspindles during meiosis II, multiple phragmoplasts, polyads of microspores, and cytoplasmic microtubule foci. These results suggest that dv is a mutation that affects MTOC organization.
Authors:
C J Staiger; W Z Cande
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Developmental biology     Volume:  138     ISSN:  0012-1606     ISO Abbreviation:  Dev. Biol.     Publication Date:  1990 Mar 
Date Detail:
Created Date:  1990-04-12     Completed Date:  1990-04-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372762     Medline TA:  Dev Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  231-42     Citation Subset:  IM    
Affiliation:
Department of Molecular and Cell Biology, University of California, Berkeley 94720.
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MeSH Terms
Descriptor/Qualifier:
Cell Division
Fluorescent Antibody Technique
Meiosis*
Metaphase*
Microtubules / physiology*,  ultrastructure
Mitotic Spindle Apparatus / ultrastructure*
Prophase*
Zea mays / genetics,  physiology*,  ultrastructure

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