Document Detail

Microtubule depolymerization modifies the incorporation of fatty acids into glycerolipids.
MedLine Citation:
PMID:  19478693     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: The influence of cytoskeletal integrity on fatty-acid (FA) metabolism is an almost unexplored field of biochemical research. This study therefore investigated the influence of cytoskeletal integrity on the incorporation of palmitate and eicosa-8,11,14-trienoate into glycerolipids of Hep G2 human hepatoma cells. MATERIAL/METHODS: Attached cultures and suspended cells were exposed to colchicine (COL, 10 microM) or dihydrocytochalasin B (DHCB, 20 microM) and supplemented with [14C]FAs bound to delipidated BSA or [14C]glycerol during 0-300 min of incubation. Various key enzymes of lipid metabolism were also determined after COL or DHCB treatment. RESULTS: Incorporation of both FAs into phospholipids (PLs) was strongly reduced by COL treatment especially in the PE and PC subfractions at short incubation times and in PS and SM for 300 min. COL also produced increased incorporation of both FAs into neutral lipids (NL), especially in TG and its precursors (MG and DG). DHCB increased the labeling into lyso-PL and reduced incorporation into PE and SM. However, this drug did not modify the [14C]NL to [14C]PL ratio. DG-acyltransferase and phosphatidate phosphohydrolase were stimulated by COL treatment. Phospholipase A2 activity was reduced significantly by COL and stimulated by DHCB treatment. CONCLUSIONS: It was demonstrated that the microtubule and microfilament network is involved in the incorporation of FAs and in its channeling to neutral lipids and phospholipids. These effects had differential characteristics depending on the type of FA involved and may have potential significance in the understanding of physiological and/or pathological processes.
Carlos A Marra; María J T de Alaniz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Medical science monitor : international medical journal of experimental and clinical research     Volume:  15     ISSN:  1643-3750     ISO Abbreviation:  Med. Sci. Monit.     Publication Date:  2009 Jun 
Date Detail:
Created Date:  2009-05-29     Completed Date:  2009-07-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9609063     Medline TA:  Med Sci Monit     Country:  Poland    
Other Details:
Languages:  eng     Pagination:  BR157-65     Citation Subset:  IM    
INIBIOLP, CONICET-UNLP, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, La Plata, Argentina.
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MeSH Terms
Cell Line, Tumor
Colchicine / pharmacology
Cytochalasin B / analogs & derivatives,  pharmacology
Fatty Acids / metabolism*
Glycerides / metabolism*
Glycerol / metabolism
Microtubules / metabolism*
Phospholipids / metabolism
Time Factors
Reg. No./Substance:
0/Fatty Acids; 0/Glycerides; 0/Phospholipids; 14930-96-2/Cytochalasin B; 39156-67-7/dihydrocytochalasin B; 56-81-5/Glycerol; 64-86-8/Colchicine

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