Document Detail

Microtubule-associated protein 2-positive cells derived from microglia possess properties of functional neurons.
MedLine Citation:
PMID:  18284917     Owner:  NLM     Status:  MEDLINE    
Microglia are believed to play an important role in the regulation of phagocytosis, neuronal survival, neuronal cell death, and inflammation. Recent studies have demonstrated that microglia are multipotential stem cells that give rise to neurons, astrocytes, and oligodendrocytes. However, the functional properties of neurons derived from microglia are poorly understood. In this study, we investigated the possibility that microglia differentiate into functional neurons. Immunocytochemical study demonstrated that microtubule-associated protein 2 (MAP2)-positive cells were derived from microglia under differentiation conditions. Intracellular Ca(2+) imaging study demonstrated that KCl caused no significant changes in [Ca(2+)](i) in microglia, whereas it caused a remarkable increase in [Ca(2+)](i) in microglia-derived cells. Furthermore, electrophysiological study demonstrated that the spike waveform, firing rate, and tetrodotoxin sensitivity of extracellular action potentials evoked by 4-aminopyridine from microglia-derived MAP2-positive cells were nearly identical to those from cultured cortical neurons. These results suggest that microglia-derived MAP2-positive cells possess properties of functional neurons.
Satoru Matsuda; Tetsuhiro Niidome; Hideki Nonaka; Yasuaki Goto; Kazuhiko Fujimura; Masaru Kato; Masaya Nakanishi; Akinori Akaike; Takeshi Kihara; Hachiro Sugimoto
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Publication Detail:
Type:  Journal Article     Date:  2008-02-20
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  368     ISSN:  1090-2104     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2008 Apr 
Date Detail:
Created Date:  2008-03-10     Completed Date:  2008-03-31     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  971-6     Citation Subset:  IM    
Department of Neuroscience for Drug Discovery, Graduate School of Pharmaceutical Sciences, Kyoto University, Yoshida-Shimoadachi-cho, Sakyo-ku, Kyoto 606-8501, Japan.
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MeSH Terms
Action Potentials
Calcium Channels / physiology
Cell Differentiation
Glial Fibrillary Acidic Protein / metabolism
Microglia / cytology*
Microtubule-Associated Proteins / metabolism*
Neurons / physiology*
Reg. No./Substance:
0/Calcium Channels; 0/Glial Fibrillary Acidic Protein; 0/Microtubule-Associated Proteins

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