Document Detail


Microtiter plate transformation using the LiAc/SS carrier DNA/PEG method.
MedLine Citation:
PMID:  17401331     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Here, we describe a protocol that has been adapted for the transformation of yeast cells in 96-well microtiter plates. This protocol can be tailored for multiple applications and is suitable for high-throughput applications. It can be completed in 2-3 h, once the yeast cells have been grown depending on the heat shock used.
Authors:
R Daniel Gietz; Robert H Schiestl
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Nature protocols     Volume:  2     ISSN:  1750-2799     ISO Abbreviation:  Nat Protoc     Publication Date:  2007  
Date Detail:
Created Date:  2007-04-02     Completed Date:  2007-06-29     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  101284307     Medline TA:  Nat Protoc     Country:  England    
Other Details:
Languages:  eng     Pagination:  5-8     Citation Subset:  IM    
Affiliation:
Department of Biochemistry and Medical Genetics, University of Manitoba, T250-770 Bannatyne Ave., Winnipeg, Manitoba R3E 0W3, Canada.
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MeSH Terms
Descriptor/Qualifier:
Acetates
Cell Culture Techniques / instrumentation,  methods*
DNA, Single-Stranded / genetics
Hot Temperature
Polyethylene Glycols
Saccharomyces cerevisiae / cytology,  genetics*,  growth & development
Transformation, Genetic*
Chemical
Reg. No./Substance:
0/Acetates; 0/DNA, Single-Stranded; 0/Polyethylene Glycols; 0/lithium acetate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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