| Microtiter plate transformation using the LiAc/SS carrier DNA/PEG method. | |
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MedLine Citation:
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PMID: 17401331 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Here, we describe a protocol that has been adapted for the transformation of yeast cells in 96-well microtiter plates. This protocol can be tailored for multiple applications and is suitable for high-throughput applications. It can be completed in 2-3 h, once the yeast cells have been grown depending on the heat shock used. |
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Authors:
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R Daniel Gietz; Robert H Schiestl |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Nature protocols Volume: 2 ISSN: 1750-2799 ISO Abbreviation: Nat Protoc Publication Date: 2007 |
Date Detail:
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Created Date: 2007-04-02 Completed Date: 2007-06-29 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 101284307 Medline TA: Nat Protoc Country: England |
Other Details:
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Languages: eng Pagination: 5-8 Citation Subset: IM |
Affiliation:
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Department of Biochemistry and Medical Genetics, University of Manitoba, T250-770 Bannatyne Ave., Winnipeg, Manitoba R3E 0W3, Canada. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acetates Cell Culture Techniques / instrumentation, methods* DNA, Single-Stranded / genetics Hot Temperature Polyethylene Glycols Saccharomyces cerevisiae / cytology, genetics*, growth & development Transformation, Genetic* |
| Chemical | |
Reg. No./Substance:
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0/Acetates; 0/DNA, Single-Stranded; 0/Polyethylene Glycols; 0/lithium acetate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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