| Microtiter broth dilution method for yeast susceptibility testing with validation by clinical outcome. | |
| | |
MedLine Citation:
|
PMID: 3771749 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
There is no ideal laboratory procedure or culture medium in current use for susceptibility testing of pathogenic yeasts. Six candidate growth media (RPMI 1640 with L-glutamine, yeast nitrogen base, Casamino Acids medium, Mueller-Hinton broth, Sabouraud dextrose broth, and minimum essential medium-Eagle salts) were screened by spectrophotometric absorbance for nucleic acid and protein. From these, two media were selected: a chemically defined growth medium (RPMI 1640 with L-glutamine) and a chemically complex medium (Casamino Acids). MICs of four antifungal agents (5-fluorocytosine, miconazole, ketoconazole, and amphotericin B) for 84 clinical isolates of various Candida species were then determined with both media in agar dilution and microtiter broth dilution systems. The resultant MICs were correlated with clinical outcome for those isolates obtained from patients treated with single antifungal agents, and susceptibility cut points were calculated. Derived MIC cut points for susceptibility were validated in a murine model of systemic candidiasis. RPMI 1640 with L-glutamine was found to have the lowest absorbance values for both nucleic acid and protein, while Casamino Acids medium was highest in both categories. We found that RPMI 1640 with L-glutamine was superior to Casamino Acids medium in the yield of MICs which correlated with actual clinical and animal outcome data. While there were no significant differences in MICs when RPMI 1640 medium was used, the microtiter broth dilution technique was superior to agar dilution in efficiency and ease of performance. We conclude that a microtiter broth system containing RPMI 1640 medium with L-glutamine is a simple, precise, and economical technique for susceptibility testing of pathogenic Candida species. We also suggest that the validation of susceptibility cut points with patient and animal outcome data make this microtiter broth system a preferential method for yeast susceptibility testing. |
| | |
Authors:
|
M Radetsky; R C Wheeler; M H Roe; J K Todd |
Related Documents
:
|
11289609 - Sucrose assimilation during early developmental stages of chicory (cichorium intybus l.... 6251039 - Sterol-content lowering action of o-chlorobenzylchloride in yeast. 17646049 - A kinetics study on the biodegradation of synthetic wastewater simulating effluent from... 19486289 - Mechanism of insoluble phosphate solubilization by pseudomonas fluorescens raf15 isolat... 4977979 - Germination of single bacterial spores. 2111119 - L-lysine production at 50 degrees c by mutants of a newly isolated and characterized me... 7605589 - Markers of dopamine depletion and compensatory response in striatum and cerebrospinal f... 15257619 - Autoxidative transformation of chiral omega6 hydroxy linoleic and arachidonic acids to ... 21218219 - Fabrication of a palladium nanoparticle/graphene nanosheet hybrid via sacrifice of a co... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Journal of clinical microbiology Volume: 24 ISSN: 0095-1137 ISO Abbreviation: J. Clin. Microbiol. Publication Date: 1986 Oct |
Date Detail:
|
Created Date: 1986-12-02 Completed Date: 1986-12-02 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 7505564 Medline TA: J Clin Microbiol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 600-6 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amphotericin B
/
pharmacology*,
therapeutic use Animals Candida / drug effects* Candidiasis / drug therapy Culture Media Flucytosine / pharmacology* Humans Ketoconazole / pharmacology*, therapeutic use Mice Miconazole / pharmacology*, therapeutic use Microbial Sensitivity Tests |
| Chemical | |
Reg. No./Substance:
|
0/Culture Media; 1397-89-3/Amphotericin B; 2022-85-7/Flucytosine; 22916-47-8/Miconazole; 65277-42-1/Ketoconazole |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Simplified enzyme-linked immunosorbent assay for specific antibodies to respiratory syncytial virus.
Next Document: New test system for identification of Aerococcus, Enterococcus, and Streptococcus species.