| Microtechnology-based three-dimensional spheroid formation. | |
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MedLine Citation:
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PMID: 23276968 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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This article highlights the current state of three-dimensional spheroid/tissue formation technologies offering a new experimental platform that is both as reproducible as conventional in vitroexperiments and highly correlated to in vivo conditions. Three-dimensional tissue exhibit higher biological functions and reflect the in vivo context more precisely than classical two-dimensional cultures or monolayers. The applications cover highly efficient drug screening, regenerative medicine and fundamental biological research. Numerous three-dimensional spheroid and tissue formation devices have been developed over the past six decades. In early studies, mass production of spheroids using shaking, and non-adhesive surfaces and scaffolds in flasks was conducted. With the emergence of micro/nano fabrication technology and fundamental understanding of micro/nano fluidics, micro/nano devices capable of forming three-dimensional spheroids in a well-controlled manner have been extensively studied. The cell species composing spheroids include hepatic cells, cancer cells, primary cells and, more recently, stem cells. Formation of hetero-spheroids composed of different cells has also been attempted to further resemble the in vivo conditions. These new trends in spheroid research are particularly highlighted in this review. |
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Authors:
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Hiroki Ota; Norihisa Miki |
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Publication Detail:
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Type: Journal Article Date: 2013-01-01 |
Journal Detail:
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Title: Frontiers in bioscience (Elite edition) Volume: 5 ISSN: 1945-0508 ISO Abbreviation: Front Biosci (Elite Ed) Publication Date: 2013 |
Date Detail:
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Created Date: 2013-01-01 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101485240 Medline TA: Front Biosci (Elite Ed) Country: United States |
Other Details:
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Languages: eng Pagination: 37-48 Citation Subset: IM |
Affiliation:
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Department of Mechanical Engineering, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama, Kanagawa 223-8522 Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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