Document Detail

Microtechnology-based three-dimensional spheroid formation.
MedLine Citation:
PMID:  23276968     Owner:  NLM     Status:  In-Data-Review    
This article highlights the current state of three-dimensional spheroid/tissue formation technologies offering a new experimental platform that is both as reproducible as conventional in vitroexperiments and highly correlated to in vivo conditions. Three-dimensional tissue exhibit higher biological functions and reflect the in vivo context more precisely than classical two-dimensional cultures or monolayers. The applications cover highly efficient drug screening, regenerative medicine and fundamental biological research. Numerous three-dimensional spheroid and tissue formation devices have been developed over the past six decades. In early studies, mass production of spheroids using shaking, and non-adhesive surfaces and scaffolds in flasks was conducted. With the emergence of micro/nano fabrication technology and fundamental understanding of micro/nano fluidics, micro/nano devices capable of forming three-dimensional spheroids in a well-controlled manner have been extensively studied. The cell species composing spheroids include hepatic cells, cancer cells, primary cells and, more recently, stem cells. Formation of hetero-spheroids composed of different cells has also been attempted to further resemble the in vivo conditions. These new trends in spheroid research are particularly highlighted in this review.
Hiroki Ota; Norihisa Miki
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Publication Detail:
Type:  Journal Article     Date:  2013-01-01
Journal Detail:
Title:  Frontiers in bioscience (Elite edition)     Volume:  5     ISSN:  1945-0508     ISO Abbreviation:  Front Biosci (Elite Ed)     Publication Date:  2013  
Date Detail:
Created Date:  2013-01-01     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101485240     Medline TA:  Front Biosci (Elite Ed)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  37-48     Citation Subset:  IM    
Department of Mechanical Engineering, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama, Kanagawa 223-8522 Japan.
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