| Microtable arrays for culture and isolation of cell colonies. | |
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MedLine Citation:
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PMID: 20644916 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cell microarrays with culture sites composed of individually removable microstructures or micropallets have proven benefits for isolation of cells from a mixed population. The laser energy required to selectively remove these micropallets with attached cells from the array depends on the microstructure surface area in contact with the substrate. Laser energies sufficient to release micropallets greater than 100 μm resulted in loss of cell viability. A new three-dimensional culture site similar in appearance to a table was designed and fabricated using a simple process that relied on a differential sensitivity of two photoresists to UV-mediated photopolymerization. With this design, the larger culture area rests on four small supports to minimize the surface area in contact with the substrate. Microtables up to 250 × 250 μm were consistently released with single 10-μJ pulses to each of the four support structures. In contrast, microstructures with a 150 × 150-μm surface area in contact with the substrate could not be reliably released at pulse energies up to 212 μJ. Cassie-Baxter wetting is required to provide a barrier of air to localize and sequester cells to the culture sites. A second asset of the design was an increased retention of this air barrier under conditions of decreased surface tension and after prolonged culture of cells. The improved air retention was due to the hydrophobic cavity created beneath the table and above the substrate which entrapped air when an aqueous solution was added to the array. The microtables proved an efficient method for isolating colonies from the array with 100% of selected colonies competent to expand following release from the array. |
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Authors:
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Jeng-Hao Pai; Wei Xu; Christopher E Sims; Nancy L Allbritton |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2010-07-20 |
Journal Detail:
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Title: Analytical and bioanalytical chemistry Volume: 398 ISSN: 1618-2650 ISO Abbreviation: Anal Bioanal Chem Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-10-28 Completed Date: 2011-02-25 Revised Date: 2011-11-01 |
Medline Journal Info:
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Nlm Unique ID: 101134327 Medline TA: Anal Bioanal Chem Country: Germany |
Other Details:
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Languages: eng Pagination: 2595-604 Citation Subset: IM |
Affiliation:
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Department of Chemistry, University of North Carolina, Chapel Hill, NC 27599, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Culture Techniques
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instrumentation,
methods Cell Separation / instrumentation, methods* Culture Media Equipment Design Hela Cells Humans Microarray Analysis / instrumentation, methods* Microscopy, Electron, Scanning Microscopy, Fluorescence Surface Properties |
| Grant Support | |
ID/Acronym/Agency:
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EB007612/EB/NIBIB NIH HHS; HG004843/HG/NHGRI NIH HHS; R01 EB007612-04/EB/NIBIB NIH HHS; R01 HG004843-02/HG/NHGRI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Culture Media |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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