Document Detail

Microsystems for the capture of low-abundance cells.
MedLine Citation:
PMID:  20636049     Owner:  NLM     Status:  MEDLINE    
Efficient selection and enumeration of low-abundance biological cells are highly important in a variety of applications. For example, the clinical utility of circulating tumor cells (CTCs) in peripheral blood is recognized as a viable biomarker for the management of various cancers, in which the clinically relevant number of CTCs per 7.5 ml of blood is two to five. Although there are several methods for isolating rare cells from a variety of heterogeneous samples, such as immunomagnetic-assisted cell sorting and fluorescence-activated cell sorting, they are fraught with challenges. Microsystem-based technologies are providing new opportunities for selecting and isolating rare cells from complex, heterogeneous samples. Such approaches involve reductions in target-cell loss, process automation, and minimization of contamination issues. In this review, we introduce different application areas requiring rare cell analysis, conventional techniques for their selection, and finally microsystem approaches for low-abundance-cell isolation and enumeration.
Udara Dharmasiri; Małgorzata A Witek; Andre A Adams; Steven A Soper
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Review    
Journal Detail:
Title:  Annual review of analytical chemistry (Palo Alto, Calif.)     Volume:  3     ISSN:  1936-1335     ISO Abbreviation:  Annu Rev Anal Chem (Palo Alto Calif)     Publication Date:  2010  
Date Detail:
Created Date:  2010-07-19     Completed Date:  2010-10-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101508602     Medline TA:  Annu Rev Anal Chem (Palo Alto Calif)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  409-31     Citation Subset:  IM    
Departments of Chemistry, Louisiana State University, Baton Rouge, 70803, USA.
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MeSH Terms
Cell Separation / methods*
Flow Cytometry
Microfluidic Analytical Techniques
Neoplasms / blood
Neoplastic Cells, Circulating
Tumor Markers, Biological / blood
Grant Support
R33 CA099246-01/CA/NCI NIH HHS
Reg. No./Substance:
0/Tumor Markers, Biological

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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