| Microsystems for the capture of low-abundance cells. | |
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MedLine Citation:
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PMID: 20636049 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Efficient selection and enumeration of low-abundance biological cells are highly important in a variety of applications. For example, the clinical utility of circulating tumor cells (CTCs) in peripheral blood is recognized as a viable biomarker for the management of various cancers, in which the clinically relevant number of CTCs per 7.5 ml of blood is two to five. Although there are several methods for isolating rare cells from a variety of heterogeneous samples, such as immunomagnetic-assisted cell sorting and fluorescence-activated cell sorting, they are fraught with challenges. Microsystem-based technologies are providing new opportunities for selecting and isolating rare cells from complex, heterogeneous samples. Such approaches involve reductions in target-cell loss, process automation, and minimization of contamination issues. In this review, we introduce different application areas requiring rare cell analysis, conventional techniques for their selection, and finally microsystem approaches for low-abundance-cell isolation and enumeration. |
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Authors:
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Udara Dharmasiri; Małgorzata A Witek; Andre A Adams; Steven A Soper |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Review |
Journal Detail:
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Title: Annual review of analytical chemistry (Palo Alto, Calif.) Volume: 3 ISSN: 1936-1335 ISO Abbreviation: Annu Rev Anal Chem (Palo Alto Calif) Publication Date: 2010 |
Date Detail:
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Created Date: 2010-07-19 Completed Date: 2010-10-28 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101508602 Medline TA: Annu Rev Anal Chem (Palo Alto Calif) Country: United States |
Other Details:
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Languages: eng Pagination: 409-31 Citation Subset: IM |
Affiliation:
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Departments of Chemistry, Louisiana State University, Baton Rouge, 70803, USA. udharm1@tigers.lsu.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Separation
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methods* Flow Cytometry Humans Microfluidic Analytical Techniques Neoplasms / blood Neoplastic Cells, Circulating Tumor Markers, Biological / blood |
| Grant Support | |
ID/Acronym/Agency:
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R33 CA099246-01/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Tumor Markers, Biological |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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