| Microstructured thin peptide-polymer films that spatially control the surface-attachment of living cells. | |
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MedLine Citation:
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PMID: 21374577 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Purpose: The integration of living cells into artificial microdevices requires close control of the interfacial interactions. Functional polymer coatings, such as peptide-polymer monolayers, can be used to guide the specific adhesion of living cells on surfaces in a spatially controlled fashion. ?Methods: Silicon surfaces were modified with a protein repellent polymer film composed of poly(dimethylacrylamide) (PDMAA) attached to the peptide cell-recognition motif GRGDSP and a subsequent PDMAA backfill. Microstructuring was achieved through a photolithographical process using surface-bound photoreactive benzophenone. Cell adhesion assays with human fibroblasts were conducted to study the capabilities of this approach to induce a directed outgrowth of living cells and to confine cell colony sizes to single cell arrays. ?Results: Human fibroblasts follow the chemically imprinted microstructures on peptide-polymer coated substrates. Lines of GRGDSP-PDMAA with a width of 10 µm induce a highly elongated cell shape whereas round spots with a diameter of 50 µm support only single cells per spot. Starting at a center-to-center distance of 100 µm between single peptide-polymer spots, cells are able to "bridge" non-adhesive PDMAA areas. ?Conclusions: Peptide-polymer monolayers can direct the outgrowth and restrict the cell colony size through a variation of the imprinted chemical microstructures. |
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Authors:
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Sönke Petersen; Markus Biesalski |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-3-3 |
Journal Detail:
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Title: The International journal of artificial organs Volume: - ISSN: 1724-6040 ISO Abbreviation: - Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2011-3-4 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7802649 Medline TA: Int J Artif Organs Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Ernst-Berl-Institute of Technical and Macromolecular Chemistry, Technical University of Darmstadt, Darmstadt - Germany. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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