Document Detail


Microstructured thin peptide-polymer films that spatially control the surface-attachment of living cells.
MedLine Citation:
PMID:  21374577     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Purpose: The integration of living cells into artificial microdevices requires close control of the interfacial interactions. Functional polymer coatings, such as peptide-polymer monolayers, can be used to guide the specific adhesion of living cells on surfaces in a spatially controlled fashion. ?Methods: Silicon surfaces were modified with a protein repellent polymer film composed of poly(dimethylacrylamide) (PDMAA) attached to the peptide cell-recognition motif GRGDSP and a subsequent PDMAA backfill. Microstructuring was achieved through a photolithographical process using surface-bound photoreactive benzophenone. Cell adhesion assays with human fibroblasts were conducted to study the capabilities of this approach to induce a directed outgrowth of living cells and to confine cell colony sizes to single cell arrays. ?Results: Human fibroblasts follow the chemically imprinted microstructures on peptide-polymer coated substrates. Lines of GRGDSP-PDMAA with a width of 10 µm induce a highly elongated cell shape whereas round spots with a diameter of 50 µm support only single cells per spot. Starting at a center-to-center distance of 100 µm between single peptide-polymer spots, cells are able to "bridge" non-adhesive PDMAA areas. ?Conclusions: Peptide-polymer monolayers can direct the outgrowth and restrict the cell colony size through a variation of the imprinted chemical microstructures.
Authors:
Sönke Petersen; Markus Biesalski
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-3-3
Journal Detail:
Title:  The International journal of artificial organs     Volume:  -     ISSN:  1724-6040     ISO Abbreviation:  -     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-3-4     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7802649     Medline TA:  Int J Artif Organs     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Ernst-Berl-Institute of Technical and Macromolecular Chemistry, Technical University of Darmstadt, Darmstadt - Germany.
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