Document Detail


Microstructure of anaerobic granules bioaugmented with Desulfitobacterium frappieri PCP-1.
MedLine Citation:
PMID:  12147505     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Oligonucleotide probes were used to study the structure of anaerobic granular biofilm originating from a pentachlorophenol-fed upflow anaerobic sludge bed reactor augmented with Desulfitobacterium frappieri PCP-1. Fluorescence in situ hybridization demonstrated successful colonization of anaerobic granules by strain PCP-1. Scattered microcolonies of strain PCP-1 were detected on the biofilm surface after 3 weeks of reactor operation, and a dense outer layer of strain PCP-1 was observed after 9 weeks. Hybridization with probes specific for Eubacteria and Archaea probes showed that Eubacteria predominantly colonized the outer layer, while Archaea were observed in the granule interior. Mathematical simulations showed a distribution similar to that observed experimentally when using a specific growth rate of 2.2 day(-1) and a low bacterial diffusion of 10(-7) dm(2) day(-1). Also, the simulations showed that strain PCP-1 proliferation in the outer biofilm layer provided excellent protection of the biofilm from pentachlorophenol toxicity.
Authors:
M Lanthier; B Tartakovsky; R Villemur; G DeLuca; S R Guiot
Related Documents :
21625875 - Screening of staphylococcus aureus nasal strains isolated from medical students for tox...
16353125 - Influence of sub-inhibitory concentrations of antimicrobial agents on biofilm formation...
17226455 - Genetic control of peltate glandular trichome formation in perilla frutescens.
10496895 - Quorum sensing-dependent regulation and blockade of exoprotease production in aeromonas...
9090815 - Structure of the o2 antigen of stenotrophomonas (xanthomonas or pseudomonas) maltophilia.
7788675 - In vitro activity of several antimicrobial agents against neisseria gonorrhoeae and com...
Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  68     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2002 Aug 
Date Detail:
Created Date:  2002-07-30     Completed Date:  2002-10-17     Revised Date:  2013-06-09    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4035-43     Citation Subset:  IM    
Affiliation:
INRS-Institut Armand-Frappier, Laval, Québec, Canada H7V 1B7.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Anaerobiosis
Archaea / genetics
Bacteria / genetics
Biodegradation, Environmental
Biofilms / growth & development*
Bioreactors*
DNA, Ribosomal / analysis
Genes, rRNA
Image Processing, Computer-Assisted
In Situ Hybridization, Fluorescence
Models, Biological
Oligonucleotide Probes
Pentachlorophenol / metabolism*
Peptococcaceae / genetics,  growth & development*,  metabolism
Sequence Analysis, DNA
Sewage / microbiology*
Chemical
Reg. No./Substance:
0/DNA, Ribosomal; 0/Oligonucleotide Probes; 0/Sewage; 87-86-5/Pentachlorophenol
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  The diversity of Phaseolus-nodulating rhizobial populations is altered by liming of acid soils plant...
Next Document:  Ability of nonpathogenic Fusarium oxysporum strain Fo47 to induce resistance against Pythium ultimum...