Document Detail

Microsporidia and Candida spores: their discrimination by Calcofluor, trichrome-blue and methylene-blue combination staining.
MedLine Citation:
PMID:  10929147     Owner:  NLM     Status:  MEDLINE    
Faeces of immunocompromised patients are often contaminated with the chitin-containing spores of microsporidia and Candida, which exclude the use of the chitin-specific fluorescent brightener Calcofluor white M2R for the identification of microsporidian spores. We developed a combination staining of Calcofluor white M2R with modified trichrome-blue staining and subsequent methylene-blue incubation which permits discrimination between these two types of spores. As a basis for diagnosis, a difference in the fluorescence pattern (365-440 nm) is combined with a difference in the light microscopic staining pattern. Under fluorescence conditions microsporidia spores have a spotted, brilliant white Calcofluor fluorescence and can easily be identified, while Candida spores show a reddish purple colour. Under the light microscope microsporidian spores show a light red colour with nonstained vacuole spots or strips in contrast to the yeast spores with their red-brown colour. This combination technique offers a highly specific means for the diagnosis of microsporidia spores in faeces.
J Schottelius; E M Kuhn; R Enriquez
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Tropical medicine & international health : TM & IH     Volume:  5     ISSN:  1360-2276     ISO Abbreviation:  Trop. Med. Int. Health     Publication Date:  2000 Jun 
Date Detail:
Created Date:  2000-08-22     Completed Date:  2000-08-22     Revised Date:  2006-11-07    
Medline Journal Info:
Nlm Unique ID:  9610576     Medline TA:  Trop Med Int Health     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  453-8     Citation Subset:  IM    
Department of Parasitology, Bernhard-Nocht-Institute for Tropical Medicine, Hamburg, Germany.
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MeSH Terms
Azo Compounds
Candida albicans / growth & development,  isolation & purification*
Candidiasis / diagnosis
Cercopithecus aethiops
Eosine Yellowish-(YS)
Feces / microbiology
Fluorescent Dyes*
Immunocompromised Host
Macaca mulatta
Methyl Green
Methylene Blue
Microsporida / growth & development,  isolation & purification*
Microsporidiosis / diagnosis
Sensitivity and Specificity
Spores / isolation & purification
Staining and Labeling / standards
Vero Cells
Reg. No./Substance:
0/Azo Compounds; 0/Benzenesulfonates; 0/Fluorescent Dyes; 0/trichrome stain; 17372-87-1/Eosine Yellowish-(YS); 4404-43-7/C.I. Fluorescent Brightening Agent 28; 61-73-4/Methylene Blue; 82-94-0/Methyl Green

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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