Document Detail


Microsphere distribution in the pancreas of anesthetized rats. Alloxan stimulates the blood flow to all islets whereas glucose only affects the blood perfusion of a subgroup of islets.
MedLine Citation:
PMID:  8872526     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
CONCLUSION: Islet blood flow stimulation induced by alloxan leads to an increased blood perfusion in all islets, whereas glucose induces a flow increase only in a subgroup of islets. BACKGROUND: The aim of the present study was to investigate if an increased islet blood flow was associated with an enhanced total number of perfused islets, or whether only a subgroup of the islets increased their blood flow. METHODS: For this purpose, the whole pancreatic and islet blood flow was measured with a microsphere technique (4.5 x 10(5) microspheres/kg body wt) in anesthetized Sprague-Dawley rats. Blood flow measurements were made 3 min after an i.v. injection of 1 mL of saline, alloxan (75 mg/kg body wt), or D-glucose (300 mg/mL). RESULTS: Alloxan reduced whole pancreatic blood flow by 50%, but almost doubled islet blood flow. Glucose did not affect whole pancreatic blood flow, but increased islet blood flow 100%. Approximately 10% of the islets in control and glucose-injected rats contained microspheres, compared with about 25% in alloxan-injected rats (P < 0.001). When the islets containing a certain number of microspheres were compared, alloxan induced a homogeneous shift of the curve to the right, i.e., increased the number of islets that contained all amounts of microspheres. Glucose preferentially increased only the fraction of islets containing > or = 3 microspheres.
Authors:
L Jansson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of pancreatology : official journal of the International Association of Pancreatology     Volume:  20     ISSN:  0169-4197     ISO Abbreviation:  Int. J. Pancreatol.     Publication Date:  1996 Aug 
Date Detail:
Created Date:  1997-01-16     Completed Date:  1997-01-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8703511     Medline TA:  Int J Pancreatol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  69-74     Citation Subset:  IM    
Affiliation:
Department of Medical Cell Biology, Uppsala University, Sweden.
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MeSH Terms
Descriptor/Qualifier:
Alloxan / pharmacology*
Animals
Blood Glucose / analysis,  drug effects
Blood Pressure / drug effects
Glucose / pharmacology*
Islets of Langerhans / blood supply*,  drug effects
Male
Microspheres
Pancreas / blood supply*,  drug effects
Rats
Rats, Sprague-Dawley
Regional Blood Flow
Chemical
Reg. No./Substance:
0/Blood Glucose; 50-71-5/Alloxan; 50-99-7/Glucose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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