| Microspectroscopic measurement of the optical properties of rat liver in the visible region. | |
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MedLine Citation:
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PMID: 7932679 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Microspectroscopy is used to investigate optical properties of haemoglobin-free perfused rat liver. Visible spectra of 20 microns diameter spot size were measured in transmission and/or reflection modes as a function of the thickness (< 1200 microns) of the liver-edge. Optical density (OD) in transmission mode increased with the increasing liver thickness, whereas in reflection mode OD decreased but became almost constant above a certain thickness (c.600 microns) of the liver. The Kubelka-Munk (KM) two-flux model, with a minor modification, was applied successfully to the analysis of the changes in OD as a function of the thickness. This approach estimates the KM absorption coefficient (EKM), KM scattering coefficient (SKM) and effective penetration depth (delta eff) of the liver. The optical properties were similar to reported values, obtained with different methods. |
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Authors:
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A Seiyama; S S Chen; H Kosaka; T Shiga |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of microscopy Volume: 175 ISSN: 0022-2720 ISO Abbreviation: J Microsc Publication Date: 1994 Jul |
Date Detail:
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Created Date: 1994-11-10 Completed Date: 1994-11-10 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0204522 Medline TA: J Microsc Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 84-9 Citation Subset: IM |
Affiliation:
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Department of Physiology, Osaka University Medical School, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Liver / anatomy & histology* Male Microspectrophotometry / methods* Rats Rats, Sprague-Dawley |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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