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Microspectrophotometry of single rhabdoms in the retina of the honeybee drone (Apis mellifera male).
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MedLine Citation:
PMID:  6644268     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The relative absorption spectra of the bistable photopigment of single rhabdoms from the dorsal region of the retina of the honeybee drone were obtained using slices of retina fixed in glutaraldehyde; less accurate measurements on unfixed tissue gave difference spectra that were similar to those for fixed retinae. The method used was based on measurements of absorbance changes during saturating adaptations of the visual pigment to different monochromatic lights. It is similar to previous methods based on measurements of difference spectra amplitudes, but is simpler to use and more accurate. The predominant pigment has states that absorb maximally at 446 (rhodopsin) and 505 nm (metarhodopsin). In addition, there is a small amount of another pigment whose two states absorb maximally at approximately 340 (UV) and 460 nm.
Authors:
R B Muri; G J Jones
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of general physiology     Volume:  82     ISSN:  0022-1295     ISO Abbreviation:  J. Gen. Physiol.     Publication Date:  1983 Oct 
Date Detail:
Created Date:  1984-01-07     Completed Date:  1984-01-07     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  2985110R     Medline TA:  J Gen Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  469-96     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Bees / physiology*
Male
Photoreceptor Cells / physiology*
Retina / physiology*
Retinal Pigments / physiology
Spectrophotometry / instrumentation,  methods*
Chemical
Reg. No./Substance:
0/Retinal Pigments

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): J Gen Physiol
ISSN: 0022-1295
ISSN: 1540-7748
Publisher: The Rockefeller University Press
Article Information
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Print publication date: Day: 1 Month: 10 Year: 1983
Volume: 82 Issue: 4
First Page: 469 Last Page: 496
ID: 2228657
Publisher Id: 84063519
PubMed Id: 6644268

Microspectrophotometry of single rhabdoms in the retina of the honeybee drone (Apis mellifera male)


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