| Microsomal triglyceride transfer protein expression during mouse development. | |
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MedLine Citation:
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PMID: 10744773 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Feto-maternal transfer of lipophilic nutrients is an important factor in the normal development of the fetus and may be mediated by lipoproteins as carriers of these nutrients. Two proteins that may be important in this process are apolipoprotein B (apoB, the major structural protein of secreted lipoproteins) and microsomal triglyceride transfer protein (MTP) whose normal activity is required for the secretion of apoB-containing lipoproteins. Although no abnormalities of conception and embryonic lethality are known in humans who inherit genetic deficiencies of either of these proteins, homozygous mice bearing knockouts of either apoB or MTP show early embryonic lethality. To characterize the ontogeny of MTP expression during embryonic mouse development, we have used in situ hybridization to characterize the pattern of expression. By using microwave heating of tissue sections to optimize hybridization, we show that there is robust MTP expression in the yolk sac tissues followed by expression in the primordial liver cell nests as early as day 9 post-coitum (E9.5). Intestinal expression is detected around E12.5 and attains full adult expression patterns by E14.5. No expression in any other tissues was observed, including developing heart, kidney, placenta, and maternal decidua. Thus the pattern of MTP expression is compatible with a role in the transfer of lipophilic nutrients from the yolk sac, prior to hepatic development and to the liver, once the circulatory system has been established. |
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Authors:
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J M Shelton; M H Lee; J A Richardson; S B Patel |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of lipid research Volume: 41 ISSN: 0022-2275 ISO Abbreviation: J. Lipid Res. Publication Date: 2000 Apr |
Date Detail:
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Created Date: 2000-06-08 Completed Date: 2000-06-08 Revised Date: 2009-11-03 |
Medline Journal Info:
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Nlm Unique ID: 0376606 Medline TA: J Lipid Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 532-7 Citation Subset: IM |
Affiliation:
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Division of Endocrinology, Diabetes, and Medical Genetics, Medical University of South Carolina, 171 Ashley Avenue, Charleston, SC 29425-2222, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Carrier Proteins / genetics*, metabolism* Embryonic and Fetal Development Extraembryonic Membranes / embryology Genes, Lethal* In Situ Hybridization / methods Intestines / embryology Liver / embryology Mice Mice, Inbred ICR Mice, Mutant Strains Microsomes / metabolism* RNA, Messenger / isolation & purification Tissue Distribution |
| Chemical | |
Reg. No./Substance:
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0/Carrier Proteins; 0/RNA, Messenger; 0/microsomal triglyceride transfer protein |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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