| Microscreening toxicity system based on living magnetic yeast and gradient chips. | |
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MedLine Citation:
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PMID: 20924564 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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There is an increasing demand for easy and cost-effective methods to screen the toxicological impact of the growing number of chemical mixtures being generated by industry. Such a screening method has been developed using viable, genetically modified green fluorescent protein (GFP) reporter yeast that was magnetically functionalised and held within a microfluidic device. The GFP reporter yeast was used to detect genotoxicity by monitoring the exposure of the cells to a well-known genotoxic chemical (methyl methane sulfonate, MMS). The cells were magnetised using biocompatible positively charged PAH-stabilised magnetic nanoparticles with diameters around 15 nm. Gradient mixing was utilised to simultaneously expose yeast to a range of concentrations of toxins, and the effective fluorescence emitted from the produced GFP was measured. The magnetically enhanced retention of the yeast cells, with their facile subsequent removal and reloading, allowed for very convenient and rapid toxicity screening of a wide range of chemicals. This is the first report showing magnetic yeast within microfluidic devices in a simple bioassay, with potential applications to other types of fluorescent reporter yeast in toxicological and biomedical research. The microfluidic chip offers a simple and low-cost screening test that can be automated to allow multiple uses (adapted to different cell types) of the device on a wide range of chemicals and concentrations. |
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Authors:
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Javier García-Alonso; Rawil F Fakhrullin; Vesselin N Paunov; Zheng Shen; Joerg D Hardege; Nicole Pamme; Stephen J Haswell; Gillian M Greenway |
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Publication Detail:
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Type: Journal Article Date: 2010-10-06 |
Journal Detail:
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Title: Analytical and bioanalytical chemistry Volume: 400 ISSN: 1618-2650 ISO Abbreviation: Anal Bioanal Chem Publication Date: 2011 May |
Date Detail:
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Created Date: 2011-04-12 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101134327 Medline TA: Anal Bioanal Chem Country: Germany |
Other Details:
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Languages: eng Pagination: 1009-13 Citation Subset: IM |
Affiliation:
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Department of Biological Sciences, University of Hull, Cottingham Road, Hull, England, HU6 7RX, UK, j.garcia-alonso@nhm.ac.uk. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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