Document Detail

Microscopical analysis of Candida albicans biofilms on heat-polymerised acrylic resin after chlorhexidine gluconate and sodium hypochlorite treatments.
MedLine Citation:
PMID:  21605193     Owner:  NLM     Status:  MEDLINE    
The ability of Candida albicans to form biofilms on denture surfaces is a significant cofactor in the pathogenesis of denture stomatitis. In this study, we applied a differential staining approach and scanning electron microscopy (SEM) to analyse the effect of sodium hypochlorite and chlorhexidine gluconate on the viability, removal and morphology of C. albicans forming biofilms on denture acrylic using an in vitro model. Immediately after treatment, to distinguish live from dead C. albicans cells in the remaining biofilms, the specimens were stained differentially and analysed by confocal scanning laser microscopy. Moreover, morphological alterations of fungal cells were investigated using scanning electron microscopy. All disinfectant solutions killed all remaining fungal cells on the specimens. Interestingly, 4% chlorhexidine did not remove these cells from the acrylic resin surface whereas sodium hypochlorite solutions (1% and 2%) provided almost complete biofilm removal. Furthermore, treating the specimens with sodium hypochlorite induced cell morphology alterations, as seen in the residual fungal cells. Finally, according to our findings, it can be suggested that sodium hypochlorite solutions are the first choice as denture cleanser when compared with 4% chlorhexidine because those solutions not only killed C. albicans biofilms but also removed them from the heat-polymerised acrylic resin.
Paulo Maurício Batista da Silva; Emílio José T Rodríguez Acosta; Luciana de Rezende Pinto; Márcia Graeff; Denise Madalena P Spolidorio; Ricardo S Almeida; Vinícius Carvalho Porto
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-05-23
Journal Detail:
Title:  Mycoses     Volume:  54     ISSN:  1439-0507     ISO Abbreviation:  Mycoses     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-10-11     Completed Date:  2012-01-23     Revised Date:  2013-05-29    
Medline Journal Info:
Nlm Unique ID:  8805008     Medline TA:  Mycoses     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  e712-7     Citation Subset:  IM    
Copyright Information:
© 2011 Blackwell Verlag GmbH.
Department of Prosthodontics, Bauru Dental School, University of São Paulo, Bauru, São Paulo, Brazil.
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MeSH Terms
Acrylic Resins*
Biofilms / drug effects,  growth & development*
Candida albicans / cytology,  growth & development*
Chlorhexidine / analogs & derivatives*,  pharmacology
Dentures / microbiology
Disinfectants / pharmacology*
Hot Temperature
Microbial Viability / drug effects*
Microscopy, Confocal
Microscopy, Electron, Scanning
Sodium Hypochlorite / pharmacology*
Staining and Labeling
Reg. No./Substance:
0/Acrylic Resins; 0/Disinfectants; 55-56-1/Chlorhexidine; 7681-52-9/Sodium Hypochlorite; MOR84MUD8E/chlorhexidine gluconate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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