Document Detail


Microscope-based label-free microfluidic cytometry.
MedLine Citation:
PMID:  21263578     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
A microscope-based label-free microfluidic cytometer capable of acquiring two dimensional light scatter patterns from single cells, pattern analysis of which determines cellular information such as cell size, orientation and inner nanostructure, was developed. Finite-difference time-domain numerical simulations compared favorably with experimental scatter patterns from micrometer-sized beads and cells. The device was capable of obtaining light scattering patterns from the smallest mature blood cells (platelets) and cord blood hematopoietic stem/progenitor cells 
(CD34 + cells) and myeloid precursor cells. The potential for evaluation of cells using this label-free microfluidic cytometric technique was discussed.
Authors:
Xuantao Su; Sean E Kirkwood; Manisha Gupta; Leah Marquez-Curtis; Yuanyuan Qiu; Anna Janowska-Wieczorek; Wojciech Rozmus; Ying Y Tsui
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Optics express     Volume:  19     ISSN:  1094-4087     ISO Abbreviation:  Opt Express     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2011-01-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101137103     Medline TA:  Opt Express     Country:  United States    
Other Details:
Languages:  eng     Pagination:  387-98     Citation Subset:  IM    
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