|Microsatellites grant more stable flanking genes.|
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|PMID: 23035963 Owner: NLM Status: MEDLINE|
|BACKGROUND: Microsatellites, or simple sequence repeats (SSRs), are DNA sequences that include tandem copies of specific sequences no longer than six bases. SSRs are ubiquitous in all genomes and highly mutable.
PRESENTATION OF THE HYPOTHESIS: Results from previous studies suggest that flanking regions of SSR are exhibit high stability in a wide range of organisms. We hypothesized that the SSRs ability to discard weak DNA polymerases could be responsible for this unusual stability. . When the weak polymerases are being decayed over SSRs, the flanking sequences would have higher opportunity to be replicated by more stable DNA polymerases. We present evidence of the molecular basis of our hypothesis.
TESTING THE HYPOTHESIS: The hypothesis could be tested by examining the activity of DNA polymerase during and after a number of PCRs. The PCR reactions should be run with the same SSR locus possessing differences in the SSR length. The hypothesis could also be tested by comparing the mutational rate of a transferred gene between two transformations. The first one has a naked T-DNA (transferred DNA), while the second one has the same T-DNA flanked with two SSRs.
IMPLICATIONS OF THE HYPOTHESIS: In any transformation experiment, flanking the T-DNA fragment with SSR sequences would result in more stably transferred genes. This process would decrease the unpredictable risks that may occur because of the mutational pressure on this foreign segment.
|Reem Joukhadar; Abdulqader Jighly|
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|Type: Journal Article Date: 2012-10-05|
|Title: BMC research notes Volume: 5 ISSN: 1756-0500 ISO Abbreviation: BMC Res Notes Publication Date: 2012|
|Created Date: 2012-12-10 Completed Date: 2013-05-23 Revised Date: 2013-07-11|
Medline Journal Info:
|Nlm Unique ID: 101462768 Medline TA: BMC Res Notes Country: England|
|Languages: eng Pagination: 556 Citation Subset: IM|
|University of Aleppo, Aleppo, Syria.|
|APA/MLA Format Download EndNote Download BibTex|
DNA / genetics, metabolism
DNA, Bacterial / genetics
DNA-Directed DNA Polymerase / chemistry, metabolism*
Genes / genetics*
Genome / genetics
Microsatellite Repeats / genetics*
Phytophthora / genetics
Plants / genetics
Polymerase Chain Reaction
Protein Structure, Secondary
|0/DNA, Bacterial; 0/T-DNA; 9007-49-2/DNA; EC 126.96.36.199/DNA-Directed DNA Polymerase|
Journal ID (nlm-ta): BMC Res Notes
Journal ID (iso-abbrev): BMC Res Notes
Publisher: BioMed Central
Copyright ©2012 Joukhadar and Jighly; licensee BioMed Central Ltd.
Received Day: 2 Month: 4 Year: 2012
Accepted Day: 2 Month: 10 Year: 2012
collection publication date: Year: 2012
Electronic publication date: Day: 5 Month: 10 Year: 2012
Volume: 5First Page: 556 Last Page: 556
PubMed Id: 23035963
Publisher Id: 1756-0500-5-556
|Microsatellites grant more stable flanking genes|
|Reem Joukhadar1||Email: firstname.lastname@example.org|
|Abdulqader Jighly2||Email: email@example.com|
1University of Aleppo, Aleppo, Syria
2International Center for Agricultural Research in the Dry Areas (ICARDA), P.O. Box 5466, Aleppo, Syria
Microsatellite or simple sequence repeats (SSRs) are tandem repeated DNA sequences including tandem copies of specific sequences no longer than six bases . SSRs are ubiquitously distributed almost in all eukaryotic and prokaryotic genomes . Their abundance and the tandem repeated nature of SSRs make the loci highly mutable loci. Two mechanisms have been attributed to this phenomenon namely  unequal crossing over  and slipping of the DNA polymerase when it run over the SSR template strand allowing one strand to hybridize with one of the multi-complimentary tandem sequences on the other strand . These mechanisms results in SSR loci being highly mutable ; this unique mutational ratio creates a need to align these loci in a special way .
There are a lot of factors that affect the mutational ratio. The longer SSRs are more variable than shorter ones; however there is no threshold for slippage occurrence . It appears that SSR mutations are dependent on motif size and nucleotide content. Besides, position in genome (coding or non-coding sequences), presence on leading or lagging strand and the distance from origin of replication also affect SSR mutations . Further, fidelity of replication and repair mechanisms as well as epigenetic factors may influence repeat stability .
Despite the high mutation rate of SSRs, their flanking regions exhibit high stability even among different taxa; there is a negative correlation between SSR length and substitution rate in nearby flanking sequence [10,11]. SSRs are increasingly being used as genetic markers for a wide range of applications such as evolution and diversity studies, genetic mapping and forensic studies .
Our new hypothesis assumes that SSRs may have the power to discard weak DNA polymerases and keep the more robust ones. This selective capacity of SSRs can lead to more accurate amplification of the microsatellite flanking regions. Further, SSR slippage may occur during the decaying and the replacment of weak polymerases. The following paragraphs would present a review on the stability of SSR flanking regions in many organisms and some supportive evidence- related to the conditions of the polymerization as well as our new molecular view of how the selection is done.
The stability of the SSR flanking regions was investigated in different organisms; three Phytophthora species (algae) ; rice and bamboo ; cotton ; Brassica rapa and Arabidopsis thaliana; cowpea, mung bean and adzuki bean ; Castanea spp. ; Carya  and wheat . Similar studies have also been carried out in different animals such as fish ; Acropora ; cattle, sheep, yak, buffalo and goat ; Probarbus jullienii and gerbils ; and in human genome studies where different SSR's flanking regions associated with genes expressed in the developing nervous system were compared . In addition, flanking regions of EST-SSR loci demonstrated a common evolutionary origin of grass fungal endophytes taxa .
Recently results with pepper chloroplast genome showed that there are two sites called inverted repeat (IRa and IRb) containing high frequency of tandem repeats . In this study, most of the hotspot regions in pepper chloroplast genome seem to be relayed at the middle of the biggest fragment LSC (large single copy) –about 87 kb– which is far from the IR sites.
These studies provide indication that SSRs may help to protect their flanking regions from different kinds of mutations.
Viguera et al. reported that the DNA polymerase dissociates from the synthesize strand when the slippage occurs. We think that this dissociation support our assumption when SSRs replace weak DNA polymerases.
It has been reported that SSRs found on the lagging strand shows higher instability than those on the leading strand without any explanation . This lends credence to our assumption because of the presence of Okazaki fragments when lagging strand is being replicated. Replicating those fragments would allow more DNA polymerases to act on them leading to a higher opportunity for weaker DNA polymerases to replicate on the lagging strand.
Further, a significant increase in DNA polymerase slippage rates was reported as a result of inefficient concentrations of ions (particularly Mg ions) during DNA replication [29,31]. It has been reported that magnesium-induced the assembly of a complete DNA polymerase catalytic complex .
Kumar et al. reported that the misbalancing of dNTPs increases the mutagenesis ratio. Consistent with our hypothesis, we suggest that the long motifs with one or two types of nucleotides could cause more decayed DNA polymerases because they may create a local misbalancing of dNTPs by using limited types of nucleotides which may enhance mutation occurrences.
Figure 1 illustrates a ribbon diagram of one of the DNA polymerases (PDB code - 1BPX) . As observed, both the template (blue) and the synthetic strands (yellow) are joined with bracket-like proteomic subunit colored with red and pink. This subunit forms a pipe that fit the 3D structure of the double stranded DNA [35,36]. The structure of this subunit prevents the DNA from formation of possible secondary structures (like the loop that occurs when the DNA polymerase slips) [36,37]. While this subunit is stable, the whole protein would be stable and the synthetic strand would have less errors. After building a huge number of bases, the instability of the DNA polymerase would increase as well as the errors rate . Therefore, when the weak polymerase gets over an SSR locus, it would create a suitable environment to generate such loop because of the multi-complementary sequences. In vivo, the DNA polymerase would stop releasing double stranded DNA . Further, the helicase enzyme would still be pushing unwinded strand toward the DNA polymerase [39-41]. This would bring heavy pressure on the weak polymerase. We assumed that when polymerase slips, its domains would separate and the free end would split from the other domain causing a decayed protein and the polymerase would lose its polymerization activity. It's also observed that this slippage happens in vitro during PCR reaction [5,31] and it has previously been reported that repeats undergo deletion if replication temperature is high . Figure 2 demonstrates a diagram describing the whole process.
When trying to improve genetic modified organisms, it would be helpful to add an SSR region to the T-DNA. Flanking the transferred gene with SSRs on both sides would decrease the unpredictable risks that may occur if the foreign T-DNA fragment mutates. Microsatellites have been used in the T-DNA as a molecular marker in order to select the transgenic cucumber lines . Microsatellites of T-DNA showed high differences in their stability. For instance, the CAG repeat of the Huntington disease exposes high stability in transgenic mice . Another study showed an instable transferred microsatellite . However, none of these studies or other publications that used transgenic organisms focused their studies on the stability of microsatellite flanking regions.
Our assumption could be tested by comparing the mutation rate of a transferred gene between two cases - the control and testing cases. The first is the control case, which has the T-DNA, while the second, the testing case, has the same T-DNA flanked by two SSRs. If there were significant differences between both cases, the hypothesis might be applicable. The hypothesis could also be tested by checking the DNA polymerase activities during and after many PCRs. All reactions should be carried out for the same SSR locus with differences in the SSR length and another reaction should be done without the SSR itself.
The authors declare that they have no competing interests.
AJ and RJ discussed the hypothesis, drafted and approved the final manuscript.
The authors would like to thank Dr. Francis C. Ogbonnaya for his valuable comments and for reviewing the final draft.
|Tautz D,Hypervariability of simple sequences as a general source for polymorphic DNA markersNucl Acids ResYear: 19891765636571|
|Merkel A,Gemmell N,Detecting short tandem repeats from genome data: opening the software black boxBrief BioinformYear: 20089535536610.1093/bib/bbn02818621747|
|Chistiakov DA,Hellemans B,Haley CS,Law AS,Tsigenopoulos CS,Kotoulas G,Bertotto D,Libertini A,Volckaert FA,A microsatellite linkage map of the European sea bass Dicentrarchus labrax LGeneticsYear: 20061701821182615937133|
|Kelkar YD,Tyekucheva S,Chiaromonte F,Makova KDL,The genome-wide determinants of human and chimpanzee microsatellite evolutionGenome ResYear: 2008181303818032720|
|Ellegren H,Microsatellites: Simple sequences with complex evolutionNat Rev GenetYear: 2004543544515153996|
|Gow C,Noble JL,Rollinson D,Jones C,A High incidence of clustered microsatellite mutations revealed by parent-offpring analysis in an African freshwater snail, Bulinus forskalii (Gastropoda, Pulmonata)GeneticaYear: 2005124778310.1007/s10709-005-0204-616011005|
|Jighly A,Hamwieh A,Ogbonnaya FC,Optimization of sequence alignment for SSR (Simple Sequence Repeat) regionsBMC Res NotesYear: 2011423910.1186/1756-0500-4-23921774810|
|Leclercq S,Rivals E,Jarne P,DNA slippage occurs at Microsatellite loci without minimal threshold length in Humans: a comparative genomic approachGenome Biol EvolYear: 2010232533510.1093/gbe/evq02320624737|
|Choudhary OP,Trivedi S,Microsatellite or simple sequence repeat (SSR) instability depends on repeat characteristics during replication and repairJ Cell and Molecular BiolYear: 2010822134|
|Santibanez-Koref MF,Gangeswaran R,Hancock JM,A relationship between lengths of Microsatellites and nearby substitution rates in Mammalian GenomesMol Biol EvolYear: 200118112119212310.1093/oxfordjournals.molbev.a00375311606708|
|Sekar M,Suresh E,Kumar NS,Mayak SK,Balakrishna C,Microsatellite DNA markers, a fisheries perspectiveAquaculture Asia MagazineYear: 20091422729|
|Schlotterer C,The evolution of molecular markers just a matter of fashionNat Rev GenetYear: 20045636910.1038/nrg124914666112|
|Schena L,Cardle L,Elcooke D,Use of genome sequence data in the design and testing of SSR markers for Phytophthora speciesBMC GenomicsYear: 2008962010.1186/1471-2164-9-62019099584|
|Chen S,Lin Y,Lin C,Chen W,Yang CH,Ku H,Transferability of rice SSR markers to bambooEuphyticaYear: 2010175233310.1007/s10681-010-0159-2|
|Altaf-Khan M,Qureshi SN,Saha S,Jenkins JN,Brubaker CL,Reddy OU,Usefulness of SSR Derived from Tetraploid Gossypium spp. for Analyses of Diploid Gossypium sppJ Crop ImprovementYear: 20061612010.1300/J411v16n01_01|
|Suwabe K,Tsukazaki H,Iketani H,Hatakeyama K,Kondo M,Fujimura M,Nunome T,Fukuoka H,Hirai M,Matsumoto S,Simple sequence repeat-based comparative genomics between Brassica rapa and Arabidopsis thaliana The genetic origin of Clubroot resistanceGeneticsYear: 200617330931910.1534/genetics.104.03896816723420|
|Vir R,Bhat KV,Lakhanpaul S,Transferability of sequence tagged microsatellite sites (STMS) primers to pulse yielding taxa belonging to PhaseolaeIJIBYear: 200956266|
|Akkak A,Boccacci P,Marinoni DT,Cross-Species Amplification of Microsatellite Markers in Castanea spp And Other Related SpeciesActa Hort. (ISHS)Year: 2010866195201|
|Herrera AM,Grauke LJ,Klein P,Validation Of 14 Nuclear SSR Markers Among Different Species Of The Genus CaryaPlant & Animal Genomes XIX Conference, Town & Country Convention CenterYear: 2011P153|
|Zhang L,Sun G,Yan Z,Chen Q,Yuan Z,Lan X,Zheng Y,Liu D,Comparison of Newly Synthetic Hexaploid Wheat with Its Donors on SSR ProductsJ Genet GenomicsYear: 2007341093994610.1016/S1673-8527(07)60105-217945172|
|Zardoya R,Vollmer DM,Craddock C,Streelman JT,Karl S,Meyer A,Evolutionary conservation of microsatellite flanking regions and their use in resolving the phylogeny of cichlid fished (Pisces:Perciformes)Proc R Soc Lond BYear: 19962631589159810.1098/rspb.1996.0233|
|Tang P,Wei NV,Chen C,Wallace CC,Allen C,Comparative Study of Genetic Variability of AAT and CTGT Microsatellites in Staghorn Coral, Acropora (Scleractinia Acroporidae)Zoological StudiesYear: 2010495657668|
|Shakyawar SK,Joshi BK,Kumar D,SSR repeat dynamics in mitochondrial genomes of five domestic animal speciesBioinformationYear: 20094415816310.6026/9732063000415820198193|
|Ghiasi N,Rashid ZA,Hooshmand S,Yusoff K,Tan SG,Bhassu S,The use of locus specific microsatellite markers for detecting genetic variation in hatchery bred Probarbus jullieniiBiotechnologyYear: 2009816617010.3923/biotech.2009.166.170|
|Du X,Chen Z,Li W,Tan Y,Lu J,Zhu X,Zhao T,Dong G,Zeng L,Development of Novel Microsatellite DNA Markers by Cross-Amplification and Analysis of Genetic Variation in GerbilsJ HeredYear: 2010101671071610.1093/jhered/esq06620525768|
|Riley DE,Krieger JN,Embryonic nervous system genes predominate in searches for dinucleotide simple sequence repeats flanked by conserved sequencesGeneYear: 2009429747910.1016/j.gene.2008.09.02518952158|
|De Jong E,Guthridge KM,Spangenberg GC,Forster GW,Sequence Analysis of SSR-Flanking Regions Identifies Genome Affinities between Pasture Grass Fungal Endophyte TaxaInt J Evol BiolYear: 201110111|
|Jo YD,Park J,Kim J,Song W,Hur C,Lee Y,Kang B,Complete sequencing and comparative analyses of the pepper (Capsicum annuum L.) plastome revealed high frequency of tandem repeats and large insertiondeletions on pepper plastomePlant Cell RepYear: 20113021722910.1007/s00299-010-0929-220978766|
|Viguera E,Canceill D,Ehrlich SD,Replication slippage involves DNA polymerase pausing and dissociationJ Mol BiolYear: 2001201025872595|
|Wang G,Vasquez KM,Models for chromosomal replication-independent non-B DNA structure-induced genetic instabilityMol CarcinogYear: 200948428629810.1002/mc.2050819123200|
|Viguera E,Canceill D,Ehrlich SD,In vitro Replication Slippage by DNA Polymerases from Thermophilic OrganismsJ Mol BiolYear: 2001312232333310.1006/jmbi.2001.494311554789|
|Batra VK,Beard WA,Shock DD,Krahn JM,Pedersen LC,Wilson SH,Magnesium-induced assembly of catalytic complexStructureYear: 20061475776610.1016/j.str.2006.01.01116615916|
|Kumar D,Abdulovic AL,Viberg J,Nilsson AK,Kunkel TA,Chabes A,Mechanisms of mutagenesis in vivo due to imbalanced dNTP poolsNucl Acids ResYear: 20103911220805246|
|Sawaya MR,Prasad R,Wilson SH,Kraut J,Pelletier H,Crystal structures of human DNA polymerase beta complexed with gapped and nicked DNA: evidence for an induced fit mechanismBiochemistryYear: 199736112051121510.1021/bi97038129287163|
|Ollis DL,Brick P,Hamlin R,Xuong NG,Steitz TA,Structure of large fragment of Escherichia coli DNA polymerase I complexed with dTMPNatureYear: 198531376276610.1038/313762a03883192|
|Steitz TA,A mechanism for all polymerasesNatureYear: 199839123123210.1038/345429440683|
|Hubscher U,Spadari S,Villani G,Giovanni M,DNA Polymerases, DiscoveryYear: 2010Ltd: Characterization and Functions in Cellular DNA Transactions. World Scientific Publishing Co. Pte|
|Nyrén P,Enzymatic method for continuous monitoring of DNA polymerase activityAnal BiochemYear: 1987167223523810.1016/0003-2697(87)90158-82831754|
|Lopes M,Foiani M,Sogo JM,Multiple mechanisms control chromosome integrity after replication fork uncoupling and restart at irreparable UV lesionsMol CellYear: 200621152710.1016/j.molcel.2005.11.01516387650|
|Takahashi TS,Wigley DB,Walter JC,Pumps, paradoxes and ploughshares: mechanism of the MCM2-7 DNA helicaseTrends Biochem SciYear: 20053043744410.1016/j.tibs.2005.06.00716002295|
|Bochman ML,Schwacha A,The Mcm2-7 complex has in vitro helicase activityMol CellYear: 20083128729310.1016/j.molcel.2008.05.02018657510|
|Sarkar PS,Chang HC,Boudi FB,Reddy S,CTG repeats show bimodal amplification in E. coliCellYear: 199895453154010.1016/S0092-8674(00)81620-79827805|
|Zhao L,Liao F,Wang CY,Miao C,Min WA,Li DS,Cheng MAB,Yu HX,Qiang WZ,Ju WR,Gang LM,Generation of transgenic cucumbers with expression of a ten-tandem repeat long acting GLP-1 analogue and their biological function on diabetic ratsChinese Sci BullYear: 2009544658466310.1007/s11434-009-0699-9|
|Goldberg YP,Kalchman MA,Metzler M,Nasir J,Zeisler J,Graham R,Koide HB,Kusky JO,Sharp AH,Ross CA,Jirik F,Hayden MR,Absence of disease phenotype and intergenerational stability of the CAG repeat in transgenic mice expressing the human Huntington disease transcriptHum Mol GenetYear: 19965217718510.1093/hmg/5.2.1778824873|
|Adachi H,Kume A,Li M,Nakagomi Y,Niwa H,Do J,Sang C,Kobayashi Y,Doyu M,Sobue G,Transgenic mice with an expanded CAG repeat controlled by the human AR promoter show polyglutamine nuclear inclusions and neuronal dysfunction without neuronal cell deathHum Mol GenetYear: 200110101039104810.1093/hmg/10.10.103911331614|
[Figure ID: F1]
Ribbon diagram of DNA polymerase (PDB code 1BPX - Sawaya et al. [ ]). The yellow line is the synthetic DNA strand. The blue line is the template DNA strand. The pink and red subunits are the bracket-like proteomic subunits that join the DNA strands together. The orange line is the free end of the DNA polymerase.
[Figure ID: F2]
Diagram explains the mechanism of the suggested hypothesis.
Keywords: Microsatellite, Polymerase Chain Reaction (PCR), Polymerase slippage, Transformation.
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