Document Detail


Microsatellites as DNA markers in Sitka spruce.
MedLine Citation:
PMID:  24162356     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Nine microsatellite loci were found by screening a genomic DNA library of Sitka spruce (Picea sitchensis) with the four oligonucleotide probes (TG), (CAC), (GATA) and (AT). Pairs of flanking primers were generated for seven microsatellites. Five primer pairs were used to screen up to 58 Sitka spruce clones. The five loci SStg3a, SStg4, SStg4a, SStg4c and SSgataS were found to have 15, 13, 4, 3 and 6 different length alleles respectively, and in using a combination of them almost all 58 Sitka spruce genotypes could be identified. The five primer pairs were successful in amplifying DNA from two other spruce species (Picea albutilia and Picea smithiana), while only one primer pair could amplify DNA from the pine species, Pinus sylvestris and Pinus latifolia. The inheritance of microsatellites in Sitka spruce was co-dominant Mendelian.
Authors:
W T van de Ven; R J McNicol
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik     Volume:  93     ISSN:  0040-5752     ISO Abbreviation:  Theor. Appl. Genet.     Publication Date:  1996 Sep 
Date Detail:
Created Date:  2013-10-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0145600     Medline TA:  Theor Appl Genet     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  613-7     Citation Subset:  -    
Affiliation:
Department of Botany and Plant Sciences, University of California, 92521, Riverside, CA, USA.
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