Document Detail

Microsatellite markers for Amborella (Amborellaceae), a monotypic genus endemic to New Caledonia.
MedLine Citation:
PMID:  23028001     Owner:  NLM     Status:  MEDLINE    
PREMISE OF THE STUDY: Informative markers are required for assessing the diversity of Amborella trichopoda, the only species of its order, endemic to New Caledonia and considered to be the sister species to all flowering plants. Therefore, expressed sequence tag (EST)-based microsatellite markers were developed. •
METHODS AND RESULTS: Fifty-five microsatellite loci were characterized in 14896 putative unigenes, which were generated by assembling A. trichopoda ESTs from the public sequence database. Seventeen markers revealed polymorphism in 80 adult shrubs from three populations. The number of alleles per locus ranged from two to 12, with a total of 132 alleles scored. The mean expected heterozygosity per population ranged from 0.336 to 0.567. •
CONCLUSIONS: These markers offer an appropriate amount of variation to investigate genetic diversity structure, gene flow, and other conservation issues.
Valérie Poncet; Marie Couderc; Christine Tranchant-Dubreuil; Céline Gomez; Perla Hamon; Serge Hamon; Yohan Pillon; Jérôme Munzinger; Alexandre de Kochko
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-10-01
Journal Detail:
Title:  American journal of botany     Volume:  99     ISSN:  1537-2197     ISO Abbreviation:  Am. J. Bot.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-15     Completed Date:  2013-05-01     Revised Date:  2014-07-31    
Medline Journal Info:
Nlm Unique ID:  0370467     Medline TA:  Am J Bot     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e411-4     Citation Subset:  IM    
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MeSH Terms
Angiosperms / genetics*
DNA, Plant / genetics,  isolation & purification
Genetic Loci / genetics
Microsatellite Repeats / genetics*
New Caledonia
Polymorphism, Genetic
Reg. No./Substance:
0/DNA, Plant

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