Document Detail


Microsatellite frequency and size variation in the parthenogenetic parasitic wasp Venturia canescens (Gravenhorst) (Hymenoptera: Ichneumonidae).
MedLine Citation:
PMID:  10971715     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Nine genomic libraries of the parthenogenetic wasp Venturia canescens were screened for microsatellite loci. In contrast to other Hymenoptera (GT)n and not (CT)n, was the predominant repeat category found with 14 kb and 28 kb genomic DNA between loci, respectively. Mono- and trinucleotide microsatellites were rarer, occurring at frequencies between 231 kb and 589 kb of genome, whilst tetranucleotide repeats are scarce, with (ATTC)n and (CCGG)n loci occurring every 692 kb and 983 kb, respectively, and only one small imperfect (GATA)n locus and no (GACA)n loci were revealed. Over 70% of the dinucleotide, and all the trinucleotide microsatellites were small (less than eleven repeats), whilst 60% to 80% of loci were imperfect. Moreover, very few compound microsatellites and only a single association between different microsatellites were observed.
Authors:
R D Butcher; S F Hubbard; W G Whitfield
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Insect molecular biology     Volume:  9     ISSN:  0962-1075     ISO Abbreviation:  Insect Mol. Biol.     Publication Date:  2000 Aug 
Date Detail:
Created Date:  2000-10-26     Completed Date:  2000-10-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9303579     Medline TA:  Insect Mol Biol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  375-84     Citation Subset:  IM    
Affiliation:
Evolutionary and Ecological Entomology Unit, Department of Biological Sciences, University of Dundee, Dundee, Scotland, UK. r.d.j.butcher@dundee.ac.uk
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MeSH Terms
Descriptor/Qualifier:
Animals
Gene Frequency
Genes, Insect*
Microsatellite Repeats*
Parthenogenesis
Wasps / genetics*

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