Document Detail


Microsatellite alterations in free-circulating serum DNA in patients with ulcerative colitis.
MedLine Citation:
PMID:  14752228     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND/AIMS: The aim of this study was to find out whether there is free-circulating DNA in the serum of patients with ulcerative colitis and whether it is possible to establish a panel of genetic alterations which might be useful markers for diagnostic purposes, staging, or follow up. METHODS: A set of 11 microsatellite markers located on different chromosomes were analyzed in a group of 59 patients with ulcerative colitis. Radiolabeled PCR products from serum and white blood cell DNA were analyzed by autoradiography and correlated with the clinical data from these patients. RESULTS: Seven of 59 patients showed one or more microsatellite alteration(s) in their serum DNA. Six patients had an alteration in one marker, and two patients had changes in 4 markers, respectively. There was no correlation between the frequency of microsatellite alterations and the clinical data. CONCLUSION: From the panel of 11 microsatellite markers used for these studies, 6 of them seem to be well suited for the detection of ulcerative colitis-associated alterations in free circulating serum DNA. In order to increase the specificity of this assay, it is necessary to increase the number of patients to be analyzed and to use a different set of markers.
Authors:
Peter Rauh; Steffen Rickes; Michael Fleischhacker
Related Documents :
21778328 - Association between left and right atrial remodeling with atrial fibrillation recurrenc...
12190168 - Inflammatory bowel disease in children 5 years of age and younger.
21667818 - Impact of manual lymphatic drainage on hemodynamic parameters in patients with heart fa...
21346558 - D-dimer assay in egyptian patients with gaucher disease: correlation with bone and lung...
3132418 - Specific treatment of benzodiazepine overdose.
21553338 - The aetiology behind torticollis and variable spine defects in patients with müllerian ...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Digestive diseases (Basel, Switzerland)     Volume:  21     ISSN:  0257-2753     ISO Abbreviation:  Dig Dis     Publication Date:  2003  
Date Detail:
Created Date:  2004-01-30     Completed Date:  2004-05-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8701186     Medline TA:  Dig Dis     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  363-6     Citation Subset:  IM    
Copyright Information:
Copyright 2003 S. Karger AG, Basel
Affiliation:
Charité-Universitätsmedizin Berlin, Medizinische Klinik m. S. Onkologie und Haematologie, Campus Charité Mitte, Berlin, Deutschland.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Biological Markers / blood*
Colitis, Ulcerative / blood,  genetics*
DNA / blood*,  genetics
Humans
Microsatellite Repeats / genetics
Polymerase Chain Reaction
Chemical
Reg. No./Substance:
0/Biological Markers; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Clinical implications of alpha-fetoprotein expression in gastric adenocarcinoma.
Next Document:  EUK-134 reduces renal dysfunction and injury caused by oxidative and nitrosative stress of the kidne...