Document Detail

Microsatellite alterations in free-circulating serum DNA in patients with ulcerative colitis.
MedLine Citation:
PMID:  14752228     Owner:  NLM     Status:  MEDLINE    
BACKGROUND/AIMS: The aim of this study was to find out whether there is free-circulating DNA in the serum of patients with ulcerative colitis and whether it is possible to establish a panel of genetic alterations which might be useful markers for diagnostic purposes, staging, or follow up. METHODS: A set of 11 microsatellite markers located on different chromosomes were analyzed in a group of 59 patients with ulcerative colitis. Radiolabeled PCR products from serum and white blood cell DNA were analyzed by autoradiography and correlated with the clinical data from these patients. RESULTS: Seven of 59 patients showed one or more microsatellite alteration(s) in their serum DNA. Six patients had an alteration in one marker, and two patients had changes in 4 markers, respectively. There was no correlation between the frequency of microsatellite alterations and the clinical data. CONCLUSION: From the panel of 11 microsatellite markers used for these studies, 6 of them seem to be well suited for the detection of ulcerative colitis-associated alterations in free circulating serum DNA. In order to increase the specificity of this assay, it is necessary to increase the number of patients to be analyzed and to use a different set of markers.
Peter Rauh; Steffen Rickes; Michael Fleischhacker
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Digestive diseases (Basel, Switzerland)     Volume:  21     ISSN:  0257-2753     ISO Abbreviation:  Dig Dis     Publication Date:  2003  
Date Detail:
Created Date:  2004-01-30     Completed Date:  2004-05-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8701186     Medline TA:  Dig Dis     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  363-6     Citation Subset:  IM    
Copyright Information:
Copyright 2003 S. Karger AG, Basel
Charité-Universitätsmedizin Berlin, Medizinische Klinik m. S. Onkologie und Haematologie, Campus Charité Mitte, Berlin, Deutschland.
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MeSH Terms
Biological Markers / blood*
Colitis, Ulcerative / blood,  genetics*
DNA / blood*,  genetics
Microsatellite Repeats / genetics
Polymerase Chain Reaction
Reg. No./Substance:
0/Biological Markers; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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