| Microsatellite typing to trace Aspergillus flavus infections in a hematology unit. | |
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MedLine Citation:
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PMID: 20410353 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Assessing the relatedness of strains isolated from patients and their environment is instrumental in documenting the source of preventable health care-associated life-threatening Aspergillus flavus human infection clusters. The present study aimed at identifying and selecting suitable microsatellite markers for A. flavus typing. This typing scheme was then applied to investigate the A. flavus epidemiology within a hematology unit in Sfax, Tunisia. Use of a combination of five markers made it possible to discern clusters of isolates and to substantiate the genetic diversity of A. flavus within clusters. Isolates from Tunisia and Marseille, France, displayed distinct haplotypes, indicating a highly significant geographical structuring of A. flavus. The typing of clinical and environmental A. flavus isolates in a hematology unit provided insights into its hospital epidemiology. From a heterogeneous genetic background, a cluster indicative of a clonal propagation episode within the unit could be identified. In two patients with invasive aspergillosis, the same genotype was found in clinical and environmental isolates, indicating hospital-acquired colonization and infection. In further studies, this novel microsatellite typing scheme might be instrumental in illuminating important epidemiological issues about A. flavus population genetics or epidemiology, including tracing the sources and routes of transmission. |
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Authors:
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Inès Hadrich; Fattouma Makni; Ali Ayadi; Stéphane Ranque |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-04-21 |
Journal Detail:
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Title: Journal of clinical microbiology Volume: 48 ISSN: 1098-660X ISO Abbreviation: J. Clin. Microbiol. Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-06-30 Completed Date: 2010-10-08 Revised Date: 2011-07-19 |
Medline Journal Info:
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Nlm Unique ID: 7505564 Medline TA: J Clin Microbiol Country: United States |
Other Details:
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Languages: eng Pagination: 2396-401 Citation Subset: IM |
Affiliation:
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Laboratoire de Biologie Moléculaire Parasitaire et Fongique, Faculté de Médecine de Sfax, Sfax, Tunisia. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aspergillosis
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epidemiology,
microbiology* Aspergillus flavus / genetics* Cluster Analysis Environmental Microbiology Genetic Variation Hospital Units Humans Microsatellite Repeats / genetics* Sequence Analysis, DNA Tunisia / epidemiology |
| Comments/Corrections | |
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