Document Detail


Microprocedure for in situ nick translation of chromosomes.
MedLine Citation:
PMID:  1394101     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have modified the procedure of in situ nick translation to shorten the autoradiographic exposure time from 1 month to 3 days and reduce the volume of nick translation solution by a factor of at least 10. The modified procedure can be carried out on individually chosen chromosome spreads. The procedure was used on chromosome spreads of three related lines of mouse mammary epithelium (+SA, -SA, CL-S1) with different degrees of tumorigenicity. We found that the autoradiographic silver grains that are observed following in situ nick translation were often placed at the apparent junction site of chromosome translocations or at the breakpoint of chromosomal pieces. We found also that silver grains were located above double minute chromosomes, which suggests that there are active genes in double minutes.
Authors:
Z Li; H L Hosick; K Fan
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cancer genetics and cytogenetics     Volume:  62     ISSN:  0165-4608     ISO Abbreviation:  Cancer Genet. Cytogenet.     Publication Date:  1992 Sep 
Date Detail:
Created Date:  1992-11-25     Completed Date:  1992-11-25     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7909240     Medline TA:  Cancer Genet Cytogenet     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  150-3     Citation Subset:  IM    
Affiliation:
Department of Biology, University of Science and Technology, Hefei Anhui, People's Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Cell Line
Chromosome Aberrations*
Genetic Techniques*
Tumor Cells, Cultured
Grant Support
ID/Acronym/Agency:
CA-46885/CA/NCI NIH HHS

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